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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
联科生物
- 库存:
500
- 英文名:
Annexin V-APC/PI Apoptosis Kit
- 保质期:
1年
- 保存条件:
2 - 8℃避光,切勿冻存
- 规格:
30T/100T
| 规格: | 30T | 产品价格: | ¥1010.0 |
|---|---|---|---|
| 规格: | 100T | 产品价格: | ¥2090.0 |
产品简介
Annexin V(或 Annexin A5)为胞内蛋白膜联蛋白家族成员,以钙依赖的方式与磷脂酰丝氨酸(PS)结合。PS 存在于正常细胞浆膜的内层,但在凋亡早期,膜不对称性丧失,PS易位至细胞表面。荧光标记的 Annexin V 可与之特异性结合,表明该细胞为凋亡细胞。
Accutase是一种天然的酶,兼具蛋白水解酶和胶原酶的活性。但它比胰酶和胶原酶更有效,使用浓度更低,使之毒性更小、更温和。因此对细胞的损伤更小,可减少因消化处理带来的假阳性。即便长时间消化,也可保持90%以上的细胞活力。适合于各种贴壁细胞、胚胎和神经元干细胞等较敏感的细胞的消化。
产品特点
- 操作简便,15分钟即可完成染色;
- 分群清晰,结果稳定可靠;
- 独家搭配阳性质控液,补偿调节不再困难;
- 提供Accutase Enzyme,适用于贴壁细胞。
结果示例

试剂盒组分

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【求助】Annexin V-FITC/PI细胞凋亡试剂盒的使用
mmsong1987 我买了Annexin V-FITC/PI细胞凋亡试剂盒,想研究某种材料的细胞毒性,看其是否会诱导细胞凋亡。之前做过MTT之类的检测,结果显示材料基本无毒性。 由于条件限制(流式细胞仪在我们学校另一个校区)不能及时进行检测,前两天进行了一次实验,是将细胞收集后拿到测试地点再进行的荧光染色处理,检测结果显示阴性对照组没有加材料处理的细胞也多处于凋亡状态,个人猜测可能跟细胞在路上耽误的时间有关(悬浮在Annexin V结合液中
择? A:第一步考虑流式细胞仪配置,第二步考虑细胞自发荧光。对于本身有荧光的细胞(如转染了荧光蛋白基因的细胞或者药物孵育的细胞),特别是培养时间久或药物处理的细胞,一定要注意细胞代谢物和药物的荧光。一般来说,细胞代谢物的荧光会干扰 FITC/PE,所以建议用 Annexin V-APC,药物的干扰可查看药物本身的发射光谱是否会对检测选择的染料有影响。 Annexin V-APC 与 7-AAD 两种染料之间补偿干扰很小,染色操作简单,实验准确。 7-AAD 染料相比于 PI 染料粘稠度小,可减少检测
产品名称 规格 Annexin V-FITC Apoptosis Detection Kit I 100tests 产品组成成分: Annexin V Binding Buffer, 10X conc (51-66121E) Annexin V-FITC (51-65874X











