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Annexin V-iFluor 488 + PI Apop

tosis Detection Reagent/凋亡试剂盒
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  • ¥720 - 31950
  • Chamot Biotech/乔默生物
  • CM001-50D/100D/500D/1000D/2000D/5000D
  • 中国台湾
  • 2026年04月24日
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    • 英文名

      Annexin V-iFluor 488 + PI Apoptosis Detection Reagent kit

    • 保质期

      2年

    • 供应商

      乔默生物技术(上海)有限公司

    • 保存条件

      4℃避光

    • 规格

      50 rxns/100 rxns/500 rxns/1000 rxns/2000 rxns/5000 rxns

    规格:50 rxns产品价格:¥720.0
    规格:100 rxns产品价格:¥1260.0
    规格:500 rxns产品价格:¥4725.0
    规格:1000 rxns产品价格:¥7655.0
    规格:2000 rxns产品价格:¥13778.0
    规格:5000 rxns产品价格:¥31950.0
    Annexin V 是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸 PS 有高度亲和力,可通过细胞外侧暴露
    的 PS 与凋亡早期细胞胞膜结合,将 Annexin V 标记荧光染料 iFluor 488 ( E x : 4 8 8 n m , E m : 5 1 4 n m )
    利用流式细胞仪或荧光显微镜可检测细胞凋亡。PI 是一种细胞核染料,搭配 Annexin V 使用可将处于不同凋亡
    时期的细胞区分开。
     
    产品应用
    本试剂盒可应用于培养细胞凋亡检测(不推荐用于检测组织样本)
    流式细胞实验操作步骤:
    1.悬浮细胞离心(2000 rpm 离心 5 分钟)收集;贴壁细胞用不含 EDTA 的胰酶消化收集。
    2.用 PBS 洗涤细胞两次(2000 rpm 离心 5 分钟) 收集 1-5×105细胞。
    3.加入 500 μL 1×Binding Buffer 重悬细胞。
    4.加入 5 μL Annexin V-iFluor 488 混匀后加入 5 μL PI 混匀,室温避光反应 15-20 分钟。
    5.孵育后将样本置于冰上,准备进行流式检测。

    引用文献:
    1. Pterostilbene prevents AKT-ERK axis-mediated polymerization of surface fibronectin on suspended lung cancer cells independently of apoptosis and suppresses metastasis.Journal of Hematology & Oncology volume 10, Article number: 72 (2017).(IF 11.059)
    2. p53 modulates the effect of ribosomal protein S6 kinase1 (S6K1) on cisplatin toxicity in chronic myeloid leukemia cells.Pharmacological Research. 119 (2017) 443–462.(IF 5.893) 
    3. Effect of Metformin Nanoparticle-Mediated Thioredoxin Interacting Protein Expression on Oxaliplatin-Induced Peripheral Neuralgia. J Nanosci Nanotechnol. 2020 Oct 1;20(10):6123-6132.(IF 1.354)
    4. Platelets are highly efficient and efficacious carriers for tumor-targeted nano-drug delivery. Drug Delivery. 2022, VOL. 29, NO. 1, 937–949. (IF 6.419)
    5. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING. Frontiers in Pharmacology. 2022 Mar 3;13:837784. (IF 5.810)

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      图标文献和实验
      该产品被引用文献
      1. Pterostilbene prevents AKT-ERK axis-mediated polymerization of surface fibronectin on suspended lung cancer cells independently of apoptosis and suppresses metastasis.Journal of Hematology & Oncology volume 10, Article number: 72 (2017).(IF 11.059)
      2. p53 modulates the effect of ribosomal protein S6 kinase1 (S6K1) on cisplatin toxicity in chronic myeloid leukemia cells.Pharmacological Research. 119 (2017) 443–462.(IF 5.893) 
      3. Effect of Metformin Nanoparticle-Mediated Thioredoxin Interacting Protein Expression on Oxaliplatin-Induced Peripheral Neuralgia. J Nanosci Nanotechnol. 2020 Oct 1;20(10):6123-6132.(IF 1.354)
      相关实验
      • Annexin V detection protocol

        protocol - Protocol for Annexin V-FITC Apoptosis Detection Kit (ab14085): 1. Incubation of cells with Annexin V-FITC     1) Induce apoptosis by desired method.     2) Collect 1-5 x 105 cells by centrifugation.     3) Resuspend cells

      • Apoptosis Measurement by Annexin V Staining

        is associated with the condensed chromatin in apoptotic cells as a measure of apoptosis. It may appear as early as 3 h post the apoptosis inducing event and precedes the ability to detect actual DNA fragmentation. The annexin V assay has been widely accepted

      • Detection of Apoptosis in Ovarian Cells In Vitro and In Vivo Using the Annexin V-Affinity Assay

        can be detected in vitro and in vivo with fluorochrome- or biotin-labeled annexin V, a protein that binds to negatively charged phospholipids in the presence of calcium ions ( 6 , 7 ). In cells that are cultured in suspension, detection of apoptosis on the basis

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      研选
      Annexin V-iFluor 488 + PI Apoptosis Detection Reagent/凋亡试剂盒
      ¥720 - 31950