- ￥1600 - 2650
- MultiSciences
- 15.63 pg/mL - 1000 pg/mL
- 杭州
- EK182
- 2025年12月18日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
15.63 pg/mL - 1000 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
| 商品名 |
Human TNF-α ELISA Kit (人肿瘤坏死因子α (TNF-α) ELISA试剂盒) |
|---|---|
| 检测方法 |
双抗夹心法 |
| 精密度 |
板内变异系数：3.8% - 8.9%；板间变异系数：5.0% - 8.4% |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 检测样本体积 |
50 μL |
| 灵敏度 |
0.42 pg/mL |
| 检测范围 |
15.63 pg/mL - 1000 pg/mL |
| 回收率 |
89% - 97% |
| 平均回收率 |
0.93 |
| 板式 |
96孔板，可拆 |
| 保存 |
试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 |
4℃蓝冰运输 |
| 组分 |
|
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
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Human TNF-a ELISA Kit（人肿瘤坏死因子α (TNF-α) ELISA试剂盒）
￥1600 - 2650
