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- 0.39 pg/mL - 25 pg/mL
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- 2025年12月14日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
0.39 pg/mL - 25 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥3400.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥2000.0 |
| 背景介绍 | γ干扰素(IFN-γ)是可溶性二聚体细胞因子，是II型干扰素的唯一成员。它主要由自然杀伤细胞(NK)和自然杀伤T细胞(NKT)细胞分泌，在固有免疫中发挥作用；在抗原特异性免疫过程中，由CD4Th1和CD8细胞毒性T细胞分泌。IFN-γ或II型干扰素对抵抗病毒、某些细菌和原生动物感染的固有免疫和适应性免疫具有重要作用。IFN-γ是巨噬细胞重要的激活剂，也是II型主要组织相容性复合物(MHCII)表达的诱导剂。IFN-γ的异常表达和很多自身炎症和自身免疫疾病相关。除了直接抑制病毒复制，IFN-γ对免疫系统的重要性更体现在其免疫刺激和免疫调控功能。
在很多不同病理情况下包括感染、自身免疫疾病、移植排斥、过敏反应和糖尿病等，IFN-γ可作为疾病标志。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IFN-γ与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。 |
| 组分 | 包被抗IFN-γ单克隆抗体的96孔聚苯乙烯酶标板 人IFN-γ冻干标准品 IFN-γ检测抗体 标准品稀释液 辣根过氧化物酶标记的链霉亲和素 信号增强剂浓缩液 信号增强剂稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 血清血浆：50 μl 细胞培养上清：100μl |
| 灵敏度 | 0.04 pg/ml |
| 标曲图 |  |
| 标准曲线范围 | 0.39-25pg/ml |
| 回收率范围 | 85%-94% |
| 平均回收率 | 0.89 |
| 板间变异系数 | 4.7 % - 7.8% |
| 板内变异系数 | 4.3% - 5.5 % |
IFNG 分子靶点信息概述
- 分子名：IFNG, interferon gamma
- 基因家族：Interferons
IFNG 分子靶点综述
IFN-γ主要由Th1型细胞产生，属于II型细胞因子中干扰素家族中的一员，是一类分泌型糖蛋白，由143个氨基酸组成，相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生，具有调节机体免疫状态，介导细胞免疫，加强受体介导的吞噬功能，增强巨噬细胞和中性粒细胞的杀伤活性，具有抗病毒感染及抗肿瘤等生物学活性。
人 Human IFNG 分子靶点信息
- 分子名：IFNG, interferon gamma
- 别称：
- IFG
- IFI
- IFN-gamma
- IMD69
- immune interferon
- interferon, gamma
- 基因序列：NCBI_Gene: 3458
- 蛋白序列：UniProtKB: P01579
人 Human IFNG靶点分子功能（预测）
Predicted to enable cytokine activity. Involved in several processes, including positive regulation of cell death; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including positive regulation of gene expression; positive regulation of protein phosphorylation; and response to virus. Located in extracellular region. Implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Biomarker of several diseases, including acute retinal necrosis syndrome; allergic rhinitis; autoimmune disease (multiple); nephritis (multiple); and otitis media (multiple).
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- IF = 12.881 Erythrocyte Membrane-Enveloped Polymeric Nanoparticles as Nanovaccine for Induction of Antitumor Immunity against Melanoma. ACS Nano (2015)
- IF = 3.671 Evaluation of optimum conditions for Achyranthes bidentata polysaccharides encapsulated in cubosomes and immunological activity in vitro. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2017)
- IF = 6.402 Exploring the immunopotentiation of Chinese yam polysaccharide poly(lactic-co-glycolic acid) nanoparticles in an ovalbumin vaccine formulation in vivo. DRUG DELIVERY (2017)
- IF = 3.493 Fecal microbiota transplantation from chronic unpredictable mild stress mice donors affects anxiety-like and depression-like behavior in recipient mice via the gut microbiota-inflammation-brain axis. STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS (2019)
- IF = 18.027 Fluorescence-Activating and Absorption-Shifting Nanoprobes for Anaerobic Tracking of Gut Microbiota Derived Vesicles. ACS Nano (2023)
- IF = 5.511 Formulation in DDA-MPLA-TDB Liposome Enhances the Immunogenicity and Protective Efficacy of a DNA Vaccine against Mycobacterium tuberculosis Infection. Frontiers in Immunology (2018)
- IF = 8.579 Gadolinium-doped Au@prussian blue nanoparticles as MR/SERS bimodal agents for dendritic cell activating and tracking. Theranostics (2020)
- IF = 5.959 Gegen Qinlian decoction enhances the effect of PD-1 blockade in colorectal cancer with microsatellite stability by remodelling the gut microbiota and the tumour microenvironment. Cell Death & Disease (2019)
- IF = 4.38 Gene knockdown of CCR3 reduces eosinophilic inflammation and the Th2 immune response by inhibiting the PI3K/AKT pathway in allergic rhinitis mice. Scientific Reports (2022)
- IF = 1.442 Ginseng stem-leaf saponins in combination with selenium enhance immune responses to an attenuated pseudorabies virus vaccine. MICROBIOLOGY AND IMMUNOLOGY (2019)
- IF = 4.086 Ginseng Stem-Leaf Saponins in Combination with Selenium Promote the Immune Response in Neonatal Mice with Maternal Antibody. Vaccines (2020)
- IF = 9.229 Glutamine Metabolism-Regulated Nanoparticles to Enhance Chemoimmunotherapy by Increasing Antigen Presentation Efficiency. ACS Applied Materials & Interfaces (2022)
- IF = 15.804 Granulocytic Myeloid-Derived Suppressor Cells Promote the Stemness of Colorectal Cancer Cells through Exosomal S100A9. Advanced Science (2019)
- IF = 3.083 GTS-21 Reduces Inflammation in Acute Lung Injury by Regulating M1 Polarization and Function of Alveolar Macrophages. SHOCK (2019)
- IF = 4.36 Gu-Ben-Fang-Xiao decoction attenuates sustained airway inflammation by suppressing ER stress response in a murine asthma remission model of respiratory syncytial virus infection. JOURNAL OF ETHNOPHARMACOLOGY (2016)
- IF = 4.236 Gut Microbiome Influences the Efficacy of PD-1 Antibody Immunotherapy on MSS-Type Colorectal Cancer via Metabolic Pathway. Frontiers in Microbiology (2020)
- IF = 3.729 Hepatitis B virus-triggered PTEN/β-catenin/c-Myc signaling enhances PD-L1 expression to promote immune evasion. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY (2019)
- IF = 5.511 Heterologous Boost Following Mycobacterium bovis BCG Reduces the Late Persistent, Rather Than the Early Stage of Intranasal Tuberculosis Challenge Infection. Frontiers in Immunology (2018)
- IF = 4.078 HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model. CELLULAR IMMUNOLOGY (2020)
- IF = 5.195 Hydroxysafflor yellow a confers neuroprotection against acute traumatic brain injury by modulating neuronal autophagy to inhibit NLRP3 inflammasomes. JOURNAL OF ETHNOPHARMACOLOGY (2023)
- IF = 11.614 Hyperthermia based individual in situ recombinant vaccine enhances lymph nodes drainage for de novo antitumor immunity. Acta Pharmaceutica Sinica B (2022)
- IF = 8.218 IL-12p40 impairs mesenchymal stem cell-mediated bone regeneration via CD4 + T cells. CELL DEATH AND DIFFERENTIATION (2016)
- IF = 8.679 ILC2-derived IL-9 inhibits colorectal cancer progression by activating CD8+ T cells. CANCER LETTERS (2021)
- IF = 2.398 Immune-enhancing effects of polysaccharides MLN-1 from by-product of Mai-luo-ning in vivo and in vitro. FOOD AND AGRICULTURAL IMMUNOLOGY (2019)
- IF = 3.03 Immunogenicity and protective efficacy induced by an mRNA vaccine encoding gD antigen against pseudorabies virus infection. VETERINARY MICROBIOLOGY (2020)
- IF = 3.909 Immunopotentiation of Polysaccharides of Atractylodes macrocephala Koidz-loaded nanostructured lipid carriers as an adjuvant. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)
- IF = 2.985 Impaired CCR9/CCL25 signalling induced by inefficient dendritic cells contributes to intestinal immune imbalance in nonalcoholic steatohepatitis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020)
- IF = 3.998 In vivo effect of magnetic microspheres loaded with E2-a in the treatment of alveolar echinococcosis. Scientific Reports (2020)
- IF = 8.643 In Vivo MR Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with NaGdF4-TAT Nanoprobes. Small (2017)
- IF = 7.561 Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model.. Frontiers in Immunology (2021)
- IF = 7.901 Inhibition of breast cancer proliferation and metastasis by strengthening host immunity with a prolonged oxygen-generating phototherapy hydrogel. JOURNAL OF CONTROLLED RELEASE (2019)
- IF = 2.676 Inhibition of RM-1 prostate carcinoma and eliciting robust immune responses in the mouse model by using VEGF-M 2 -GnRH 3 -hinge-MVP vaccine. GENES AND IMMUNITY (2018)
- IF = 32.086 Inhibition of Tumor Metastasis by Liquid Nitrogen-Shocked Tumor Cells with Oncolytic Viruses Infection. ADVANCED MATERIALS (2023)
- IF = 11.504 Insight into the structural and immunomodulatory relationships of polysaccharides from Dendrobium officinale-an in vivo study. FOOD HYDROCOLLOIDS (2023)
- IF = 3.418 Interaction between ANXA1 and GATA-3 in Immunosuppression of CD4. MEDIATORS OF INFLAMMATION (2016)
- IF = 15.881 Intravenous Delivery of Living Listeria monocytogenes Elicits Gasdmermin-Dependent Tumor Pyroptosis and Motivates Anti-Tumor Immune Response. ACS Nano (2022)
- IF = 6.304 Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade. Cell Death & Disease (2020)
- IF = 2.886 JAK-STAT Domain Enhanced MUC1-CAR-T Cells Induced Esophageal Cancer Elimination. Cancer Management and Research (2020)
- IF = 4.927 Lipid Mediators Metabolic Chaos of Asthmatic Mice Reversed by Rosmarinic Acid. MOLECULES (2023)
- IF = 7.877 Liposomes with cyclic RGD peptide motif triggers acute immune response in mice. JOURNAL OF CONTROLLED RELEASE (2018)
- IF = 3.943 LncRNA AK085865 depletion ameliorates asthmatic airway inflammation by modulating macrophage polarization. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 2.046 Long non-coding RNA growth arrest specific-5: a potential biomarker for early diagnosis of severe asthma. Journal of Thoracic Disease (2020)
- IF = 5.578 Long-Term Immunogenicity and In Vitro Prophylactic Protective Efficacy of M. tuberculosis Fusion Protein DR2 Combined with Liposomal Adjuvant DIMQ as a Boosting Vaccine for BCG. ACS Infectious Diseases (2023)
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Human IFN-γ High Sensitivity ELISA Kit（人γ干扰素高敏 ELISA 试剂盒）
￥2000 - 3400
