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- 15.63 pg/mL - 1000 pg/mL
- 杭州
- EK180
- 2025年11月18日
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- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
15.63 pg/mL - 1000 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
| 背景介绍 | γ干扰素(IFN-γ)是可溶性二聚体细胞因子，是II型干扰素的唯一成员。它主要由自然杀伤细胞(NK)和自然杀伤T细胞(NKT)细胞分泌，在固有免疫中发挥作用；在抗原特异性免疫过程中，由CD4Th1和CD8细胞毒性T细胞分泌。IFN-γ或II型干扰素对抵抗病毒、某些细菌和原生动物感染的固有免疫和适应性免疫具有重要作用。IFN-γ是巨噬细胞重要的激活剂，也是II型主要组织相容性复合物(MHCII)表达的诱导剂。IFN-γ的异常表达和很多自身炎症和自身免疫疾病相关。除了直接抑制病毒复制，IFN-γ对免疫系统的重要性更体现在其免疫刺激和免疫调控功能。
在很多不同病理情况下包括感染、自身免疫疾病、移植排斥、过敏反应和糖尿病等，IFN-γ可作为疾病标志。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IFN-γ与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。 |
| 组分 | 包被抗人IFN-γ单克隆抗体的96孔聚苯乙烯酶标板 人IFN-γ冻干标准品 IFN-γ 检测抗体 标准品稀释液 辣根过氧化物酶标记的链霉亲和素 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 血清血浆：50 μl 细胞培养上清：100μl |
| 灵敏度 | 0.30 pg/ml |
| 标准曲线范围 | 15.63 - 1000 pg/ml |
| 回收率范围 | 83%-113% |
| 平均回收率 | 0.95 |
| 板间变异系数 | 4.5 % - 7.6 % |
| 板内变异系数 | 3.6 % -4.5% |
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- IF = 3.083 GTS-21 Reduces Inflammation in Acute Lung Injury by Regulating M1 Polarization and Function of Alveolar Macrophages. SHOCK (2019)
- IF = 4.36 Gu-Ben-Fang-Xiao decoction attenuates sustained airway inflammation by suppressing ER stress response in a murine asthma remission model of respiratory syncytial virus infection. JOURNAL OF ETHNOPHARMACOLOGY (2016)
- IF = 4.236 Gut Microbiome Influences the Efficacy of PD-1 Antibody Immunotherapy on MSS-Type Colorectal Cancer via Metabolic Pathway. Frontiers in Microbiology (2020)
- IF = 3.729 Hepatitis B virus-triggered PTEN/β-catenin/c-Myc signaling enhances PD-L1 expression to promote immune evasion. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY (2019)
- IF = 5.511 Heterologous Boost Following Mycobacterium bovis BCG Reduces the Late Persistent, Rather Than the Early Stage of Intranasal Tuberculosis Challenge Infection. Frontiers in Immunology (2018)
- IF = 4.078 HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model. CELLULAR IMMUNOLOGY (2020)
- IF = 5.195 Hydroxysafflor yellow a confers neuroprotection against acute traumatic brain injury by modulating neuronal autophagy to inhibit NLRP3 inflammasomes. JOURNAL OF ETHNOPHARMACOLOGY (2023)
- IF = 11.614 Hyperthermia based individual in situ recombinant vaccine enhances lymph nodes drainage for de novo antitumor immunity. Acta Pharmaceutica Sinica B (2022)
- IF = 8.218 IL-12p40 impairs mesenchymal stem cell-mediated bone regeneration via CD4 + T cells. CELL DEATH AND DIFFERENTIATION (2016)
- IF = 8.679 ILC2-derived IL-9 inhibits colorectal cancer progression by activating CD8+ T cells. CANCER LETTERS (2021)
- IF = 2.398 Immune-enhancing effects of polysaccharides MLN-1 from by-product of Mai-luo-ning in vivo and in vitro. FOOD AND AGRICULTURAL IMMUNOLOGY (2019)
- IF = 3.03 Immunogenicity and protective efficacy induced by an mRNA vaccine encoding gD antigen against pseudorabies virus infection. VETERINARY MICROBIOLOGY (2020)
- IF = 3.909 Immunopotentiation of Polysaccharides of Atractylodes macrocephala Koidz-loaded nanostructured lipid carriers as an adjuvant. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)
- IF = 2.985 Impaired CCR9/CCL25 signalling induced by inefficient dendritic cells contributes to intestinal immune imbalance in nonalcoholic steatohepatitis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020)
- IF = 3.998 In vivo effect of magnetic microspheres loaded with E2-a in the treatment of alveolar echinococcosis. Scientific Reports (2020)
- IF = 8.643 In Vivo MR Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with NaGdF4-TAT Nanoprobes. Small (2017)
- IF = 7.561 Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model.. Frontiers in Immunology (2021)
- IF = 7.901 Inhibition of breast cancer proliferation and metastasis by strengthening host immunity with a prolonged oxygen-generating phototherapy hydrogel. JOURNAL OF CONTROLLED RELEASE (2019)
- IF = 2.676 Inhibition of RM-1 prostate carcinoma and eliciting robust immune responses in the mouse model by using VEGF-M 2 -GnRH 3 -hinge-MVP vaccine. GENES AND IMMUNITY (2018)
- IF = 32.086 Inhibition of Tumor Metastasis by Liquid Nitrogen-Shocked Tumor Cells with Oncolytic Viruses Infection. ADVANCED MATERIALS (2023)
- IF = 11.504 Insight into the structural and immunomodulatory relationships of polysaccharides from Dendrobium officinale-an in vivo study. FOOD HYDROCOLLOIDS (2023)
- IF = 3.418 Interaction between ANXA1 and GATA-3 in Immunosuppression of CD4. MEDIATORS OF INFLAMMATION (2016)
- IF = 15.881 Intravenous Delivery of Living Listeria monocytogenes Elicits Gasdmermin-Dependent Tumor Pyroptosis and Motivates Anti-Tumor Immune Response. ACS Nano (2022)
- IF = 6.304 Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade. Cell Death & Disease (2020)
- IF = 2.886 JAK-STAT Domain Enhanced MUC1-CAR-T Cells Induced Esophageal Cancer Elimination. Cancer Management and Research (2020)
- IF = 4.927 Lipid Mediators Metabolic Chaos of Asthmatic Mice Reversed by Rosmarinic Acid. MOLECULES (2023)
- IF = 7.877 Liposomes with cyclic RGD peptide motif triggers acute immune response in mice. JOURNAL OF CONTROLLED RELEASE (2018)
- IF = 3.943 LncRNA AK085865 depletion ameliorates asthmatic airway inflammation by modulating macrophage polarization. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 2.046 Long non-coding RNA growth arrest specific-5: a potential biomarker for early diagnosis of severe asthma. Journal of Thoracic Disease (2020)
- IF = 5.578 Long-Term Immunogenicity and In Vitro Prophylactic Protective Efficacy of M. tuberculosis Fusion Protein DR2 Combined with Liposomal Adjuvant DIMQ as a Boosting Vaccine for BCG. ACS Infectious Diseases (2023)
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Human IFN-gamma ELISA Kit（人γ干扰素 ELISA试剂盒）
￥1600 - 2650
