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- 31.25 pg/mL - 2000 pg/mL
- 杭州
- EK187-96
- 2025年12月05日
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- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
31.25 pg/mL - 2000 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
| 背景介绍 | 趋化因子配体2(CCL2)，又名单核细胞趋化蛋白1(MCP-1)，是CC趋化因子家族的小分子量细胞因子。CCL2通过蛋白聚糖的粘多糖侧链锚定到内皮细胞的质膜上，主要由单核细胞、巨噬细胞和树突状细胞分泌。CCL2可招募单核细胞、记忆性T细胞和树突状细胞到组织损伤或感染引起的炎症位点。CCL2参与以单核细胞浸润为特征的一些疾病发病机制，如银屑病、类风湿性关节炎和动脉粥样硬化。CCL2参与发生在中枢神经系统的多种疾病的神经炎症过程，表现为神经元变性。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CCL2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的CCL2与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CCL2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。 |
| 组分 | 包被抗CCL2/MCP-1单克隆抗体的96孔聚苯乙烯酶标板 人CCL2/MCP-1冻干标准品 CCL2/MCP-1检测抗体 标准品稀释液 辣根过氧化物酶标记的链霉亲和素 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 50 μl |
| 灵敏度 | 2.18pg/ml |
| 标曲图 |  |
| 标准曲线范围 | 31.25-2000pg/ml |
| 回收率范围 | 95%-118% |
| 平均回收率 | 1.07 |
| 板间变异系数 | 5.3 % - 7.1% |
| 板内变异系数 | 3.8% -8.8 % |
CCL2 分子靶点信息概述
- 分子名：CCL2, C-C motif chemokine ligand 2
- 基因家族：Chemokine ligands
- 别名：MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
- 曾用名：SCYA2
- 全称：monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2
CCL2 分子靶点综述
趋化因子配体2(CCL2)，又名单核细胞趋化蛋白1(MCP-1)，是CC趋化因子家族的小分子量细胞因子。CCL2通过蛋白聚糖的粘多糖侧链锚定到内皮细胞的质膜上，主要由单核细胞、巨噬细胞和树突状细胞分泌。CCL2可招募单核细胞、记忆性T细胞和树突状细胞到组织损伤或感染引起的炎症位点。CCL2参与以单核细胞浸润为特征的一些疾病发病机制，如银屑病、类风湿性关节炎和动脉粥样硬化。CCL2参与发生在中枢神经系统的多种疾病的神经炎症过程，表现为神经元变性。
人 Human CCL2 分子靶点信息
- 分子名：CCL2, C-C motif chemokine ligand 2
- 别称：
- C-C motif chemokine 2
- chemokine (C-C motif) ligand 2
- GDCF-2
- HC11
- HSMCR30
- MCAF
- MCP-1
- MCP1
- MGC9434
- monocyte chemoattractant protein 1
- monocyte chemoattractant protein-1
- monocyte chemotactic and activating factor
- monocyte chemotactic protein 1
- monocyte secretory protein JE
- SCYA2
- small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
- small inducible cytokine subfamily A (Cys-Cys), member 2
- small-inducible cytokine A2
- SMC-CF
- 基因序列：NCBI_Gene: 6347
- 蛋白序列：UniProtKB: P13500
人 Human CCL2靶点分子功能（预测）
Predicted to enable CCR2 chemokine receptor binding activity and chemokine activity. Involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and regulation of apoptotic process. Located in extracellular region. Implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Biomarker of several diseases, including autoimmune disease (multiple); liver disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and retinal disease (multiple).
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相关实验
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人MCP-1 ( MCP - 1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 MCP-1/CCL2单抗包被于酶标板上，标准品和样品中的 MCP-1与单抗结合，加入生物
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 猪MCP-1 ( MCP - 1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗猪 MCP-1/CCL2单抗包被于酶标板上，标准品和样品中的 MCP-1与单抗结合，加入生物
consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
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Human MCP-1/CCL2 ELISA Kit（人单核细胞趋化蛋白1/趋化因子配体2 ELISA试剂盒）
￥1600 - 2650
