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- Sino Biological
- 19796-H09B
- 2025年08月12日
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- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：NUP210蛋白, NUP210 protein
蛋白构建：A DNA sequence encoding the human NUP210 (NP_079199.2) (Leu1837-His1887) was expressed with a GST tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met
蛋白分子量：The recombinant human NUP210 consists of 279 amino acids and predicts a molecular mass of 31.7 kDa.
蛋白NP号：NP_079199.2
蛋白氨基酸序列：Leu1837-His1887
蛋白标签：N-GST
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验GST fusion protein purification GST融合蛋白纯化
spin 2',4k on bench.10)aspirate,resuspend in 25ml HBT,spin,repeat.11)pour slurry into column (Econo 1.7x20),elute to top,then with 20 column vols.of HB-T.12)elute protein in minimal volume (5-10ml)HB 5mM GT (Sigma G4251,1.5 mg/ml).13)lN2 freeze as 100ml
GST fusion protein purification--GST融合蛋白纯化
GST fusion protein purification GST融合蛋白纯化John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze
Thaw out 1 ml aliquot of fusion protein lysate. Centrifuge to remove precipitate. Incubate 1 ml lysate with 0.4 ml 50% GST beads. Incubate at 4C with mixing for 1 hour. Centrifuge 5K 2' in 1.5 ml eppendorf tubes. Wash the beads 4X with PBST
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