- ￥3000 - 3999
- Abbiotec
- 美国
- 252634
- 2026年05月27日
- WB, IHC
- Mouse
- H
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of Rab25 expressed in E. coli.
- 亚型：
Mouse IgG2a
- 形态：
Each vial contains 0.1 mg IgG in PBS pH7.4 with 0.03% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件：
Store at -20°C
- 克隆性：
单克隆
- 标记物：
见下方详细说明
- 适应物种：
H
- 保质期：
1-2年
- 抗原来源：
Purified recombinant fragment of Rab25 expressed in E. coli.
- 目录编号：
252634
- 级别：
超纯
- 库存：
大量
- 供应商：
Abbiotec
- 宿主：
Mouse
- 应用范围：
WB, IHC
- 浓度：
见详细说明信息
- 靶点：
Rab25 (3F12)
- 抗体英文名：
Rab25 (3F12) Antibody
- 抗体名：
Rab25 (3F12) Antibody
- 规格：
0.1 mg
Rab25 (3F12) Antibody产品详细介绍：
| 产品名称： | Rab25 (3F12) Antibody |
| 说明书： | 【点击查看详细说明及参考图片】 |
| 货号： | 252634 |
| 规格： | 0.1 mg |
| 产品描述： | Rab25 is a 28 kDa protein, belongs to the small GTP-ase superfamily. Members of the Ras-related superfamily of GTP binding proteins which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactios. |
| 克隆类型： | Mouse Monoclonal Antibody |
| 亚型： | Mouse IgG2a |
| 免疫原： | Purified recombinant fragment of Rab25 expressed in E. coli. |
| 适用物种： | H |
| 应用范围： | WB, IHC |
| 应用说明： | WB: 1:500-1:2000; IHC: 1:200-1:1000 |
| 别名： | Ras-related protein Rab-25; CATX-8; RAB25 |
| 形态： | Each vial contains 0.1 mg IgG in PBS pH7.4 with 0.03% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件： | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献： |
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文献和实验基 NP培养基 中胚层诱导培养基 ABC3R培养基 C1F培养基 基础培养基 Knockout DMEM DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 Penicillin-Streptomycin
/DMEM:F12 Resuspend in an appropriate volume of 10% FBS/DMEM:F12 and keep on ice Add 50μl cell suspension to 50μl 10% FBS/DMEM:F12 containing appropriate amount of antibody to give the final concentration listed below and incubate for 30min
穹顶状培养，以促进类器官生长。 1.细胞制备 ①组织样本置于含高级DMEM/F12和普里莫星的50mL离心管中，4℃保存直至分离。为确保最佳效果，30mg组织需在24h内解离，以防止细胞死亡和冻融损伤。 ②为每份组织样本制备新鲜消化液：在15mL离心管中加入5mL高级DMEM/F12，再加入5mg/mLII型胶原酶和10μMY27632，混匀后4℃保存备用。 ③将组织转移至10cm培养皿中。用两把手术刀将组织切成约1mm³的小块，用移液管加入5mL消化液。 ④将培养皿中的内容物转移至15
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