- ￥5600
- GeneTex
- 美国
- GTX82785
- 2025年07月12日
- WB, ICC/IF, IHC-P, FACS, ELISA
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of Rab25 expressed in E. Coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX82785
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, ELISA
- 靶点：
Rab25
- 抗体英文名：
Rab25 antibody [3F12]
- 抗体名：
Rab25 抗体 [3F12]
- 规格：
100 μl
WB analysis of MCF-7 (1), T47D (2) and GC7901 (3) cell lysate using GTX82785 Rab25 antibody [3F12].
ICC/IF analysis of A549 cells using GTX82785 Rab25 antibody [3F12].
Green : Rab25
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
IHC-P analysis of esophagus tissue (left) and human lung cancer (right) using GTX82785 Rab25 antibody [3F12].
FACS analysis of NIH3T3 cells using GTX82785 Rab25 antibody [3F12].
Green : Rab25
Purple : negative control
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文献和实验Qiu S et al., Biomed Pharmacother 2019 (PMID:30551439)
/DMEM:F12 Resuspend in an appropriate volume of 10% FBS/DMEM:F12 and keep on ice Add 50μl cell suspension to 50μl 10% FBS/DMEM:F12 containing appropriate amount of antibody to give the final concentration listed below and incubate for 30min
） 3. BSA 4. DMEM 5. FCS 6. Antibiotic 7. Heparin 8. F12medium 9. Isoflourane 10. Ethonol 11. Dynabeads 12. anti-CD31antibody（BDPharmingen，catalognumber：550274） 13. M199 14. gelatin 二、设备 1. 离心机 2. 无菌培养橱
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
bovine serum (FBS; Invitrogen, cat. no. 16000‐044) in DMEM/F12 medium (chilled to 4°C) 37°C incubator
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