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- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
联科生物
- 检测范围:
62.5 pg/mL - 4000 pg/mL
- 检测方法:
双抗夹心法
- 应用:
ELISA
- 适应物种:
Human
- 标记物:
HRP
- 样本:
血清,血浆,细胞培养上清及其他生物学样本
- 规格:
96T/48T
| 规格: | 96T | 产品价格: | ¥2650.0 |
|---|---|---|---|
| 规格: | 48T | 产品价格: | ¥1600.0 |
| 背景介绍 | 肝细胞生长因子(HGF)是旁分泌性细胞生长、运动和形态发生的因子,由间充质细胞分泌产生,可作为大部分上皮来源细胞的多功能细胞因子。HGF以单链形式存在,是一个带有29个氨基酸信号序列和25个氨基酸前体序列的728个氨基酸残基前肽。HGF结合原癌基因c-Met受体后就会激活酪氨酸激酶信号级联,进而调节细胞的生长、运动和形态发生。它在血管生成、肿瘤发生和组织再生中起核心作用。目前正在测试人HGF质粒DNA治疗心肌细胞是否可作为冠状动脉疾病和心肌梗死后心脏损伤的一种潜在治疗。高水平的HGF与与快速康复有关。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人HGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的HGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中HGF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570-630 nm)测定吸光度值。 |
| 组分 | 包被抗HGF单克隆抗体的96孔聚苯乙烯酶标板 人HGF冻干标准品 HGF检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆,细胞培养上清及其他生物学样本 |
| 板式 | 96孔板,可拆 |
| 保存 | 试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 20 μl |
| 灵敏度 | 1.99 pg/ml |
| 标曲图 | ![]() |
| 标准曲线范围 | 62.5-4000 pg/ml |
| 回收率范围 | 85 %-108 % |
| 平均回收率 | 0.95 |
| 板间变异系数 | 3.1 %-8.3 % |
| 板内变异系数 | 4.3 %-6.5 % |
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Human HGF ELISA Kit(人肝细胞生长因子ELISA试剂盒)
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