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- 技术资料
- 库存:
99
- 供应商:
联科生物
- 检测范围:
15.63 pg/mL - 1000 pg/mL
- 检测方法:
双抗夹心法
- 应用:
ELISA
- 适应物种:
Human
- 标记物:
HRP
- 样本:
血清,血浆,细胞培养上清及其他生物学样本
- 规格:
96T/48T
| 规格: | 96T | 产品价格: | ¥2650.0 |
|---|---|---|---|
| 规格: | 48T | 产品价格: | ¥1600.0 |
| 背景介绍 | 干扰素(IFNs)是宿主细胞在响应病原体(如病毒、细菌、寄生虫或者肿瘤细胞)时合成和释放的一组信号蛋白。在通常情况下,病毒感染的细胞会释放干扰素,使周围的细胞提高其抗病毒防御能力。基于受体类型,人类干扰素可分为3大类型:I型干扰素,包括IFN-α、IFN-β、IFN-ε、IFN-κ和IFN-ω,II型干扰素(在人类中称为IFN-γ)和III型干扰素。
IFN-β通过信号通路STAT1和STAT2上调和下调多种基因,其中,大部分基因参与抗病毒免疫反应。IFN-β在诱导广泛的非特异性抗病毒感染中起重要作用,它还影响细胞增殖并调节免疫应答。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IFN-β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IFN-β与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IFN-β的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570-630 nm)测定吸光度值。 |
| 组分 | 包被抗IFN-β单克隆抗体的96孔聚苯乙烯酶标板 人IFN-β冻干标准品 IFN-β检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆,细胞培养上清及其他生物学样本 |
| 板式 | 96孔板,可拆 |
| 保存 | 试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 50μl |
| 灵敏度 | 3.19 pg/ml |
| 标曲图 | ![]() |
| 标准曲线范围 | 15.63-1000 pg/ml |
| 回收率范围 | 79 %-95 % |
| 平均回收率 | 0.88 |
| 板间变异系数 | 3.6 %-7.9 % |
| 板内变异系数 | 4.5 %-5.0 % |
概述人 IFNB1, IFN-β, IFNB 靶点信息
IFNB1 分子靶点信息概述
- 分子名:IFNB1, interferon beta 1
- 基因家族:Interferons
- 别名:IFB; IFF
- 曾用名:IFNB
- 全称:interferon, beta 1, fibroblast
IFNB1 分子靶点综述
干扰素(IFNs)是宿主细胞在响应病原体(如病毒、细菌、寄生虫或者肿瘤细胞)时合成和释放的一组信号蛋白。在通常情况下,病毒感染的细胞会释放干扰素,使周围的细胞提高其抗病毒防御能力。基于受体类型,人类干扰素可分为3大类型:I型干扰素,包括IFN-α、IFN-β、IFN-ε、IFN-κ和IFN-ω,II型干扰素(在人类中称为IFN-γ)和III型干扰素。IFN-β通过信号通路STAT1和STAT2上调和下调多种基因,其中,大部分基因参与抗病毒免疫反应。IFN-β在诱导广泛的非特异性抗病毒感染中起重要作用,它还影响细胞增殖并调节免疫应答。





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Human IFN-β ELISA Kit(人干扰素-β ELISA试剂盒)
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