- ￥1080 - 12880
- Sino Biological
- 北京
- 51048-M01H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse GM-CSF / CSF2 Protein (Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg/500.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥1980.0 |
| 规格： | 500.00 µg | 产品价格： | ￥12880.0 |
蛋白名称：GM-CSF蛋白, GM-CSF protein
蛋白构建：A DNA sequence encoding the mouse CSF2 (P01587) (Ala18-Lys141) was was expressed with the fused Fc region of human IgG1 at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 0.02-0.1 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu
蛋白分子量：The recombinant mouse CSF2/Fc comprises 384 amino acids and has a predicted molecular mass of 42.5 kDa. The apparent molecular mass of the protein is approximately 44-52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：P01587
蛋白氨基酸序列：Ala18-Lys141
蛋白标签：N-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Li Y, et al. Rapid surface display of mRNA antigens by bacteria-derived outer membrane vesicles for a personalized tumor vaccine.Advanced materials (Deerfield Beach, Fla.), PubMed ID: 35315546
3, Gao X, et al. mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine.Bio-protocol, PubMed ID: 37456344
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
L? Protein A—只与 Fc 区域结合 Protein G—结合 Fc 区域和部分轻链 Protein A/G—重组蛋白，兼具 Protein A 和 Protein G 的结合位点 Protein L—结合仅限于那些含有 κ 轻链的抗体 省事且通用可选择 Protein A/G，也可根据结合力表格进行选择，详见资源信息部分 3、基质—琼脂糖还是磁珠？ 琼脂糖 磁珠 优点 简单易用，无需辅助设备 操作简单 孔径大，直径大（50-150
Purification of Antiserum or Ascites by Protein A/G Chromatography
A and protein G bind about 20 mg of IgG per ml of conjugated agarose. Therefore, the binding capacity of a 10 ml (5 ml) column is 200 mg (100 mg) of IgG. A high-titer rabbit antiserum has roughly 5 mg/ml of IgG, mouse ascites has roughly 10 mg/ml of IgG
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