GelRedTM Nucleic Acid Gel Stai

GelRed^TM is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed without changing your existing imaging system.

GelRed^TM can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.

A series of safety tests have confirmed that GelRed^TM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed/GelGreen Safety Report here.

Biotium offers GelRed^TM 10,000X solution in water (cat#41003), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelRed^TM 10,000X solution in DMSO for established users of GelRed^TM in DMSO who do not wish to change their laboratory protocols. The performance and stability of GelRed^TM 10,000X is comparable in both the water and DMSO formulations. For your convenience, we also offer ready-to-use GelRed^TM 3X in water (cat#41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10mL (cat#41003-1).