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- Biotium
- 41001
- 美国
- 2025年07月16日
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- 库存：
大量
- 供应商：
上海研卉生物
- 英文名：
GelRedTM Nucleic Acid Gel Stain, 3X in water
- 规格：
4L
Description:
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GelRedTM is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed without changing your existing imaging system. GelRedTM can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning. A series of safety tests have confirmed that GelRedTM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed/GelGreen Safety Report here.
Biotium offers GelRedTM 10,000X solution in water (cat#41003), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelRedTM 10,000X solution in DMSO (cat#41002) for established users of GelRedTM in DMSO who do not wish to change their laboratory protocols. The performance and stability of GelRedTM 10,000X is comparable in both the water and DMSO formulations.For your convenience, we also offer ready-to-use GelRedTM 3X in water (cat#41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10mL (cat#41003-1).
Figure 3. Excitation and emission spectra of GelRed and GelGreen in the presence of DNA in PBS buffer
Note: *GelRed and its uses are covered by pending US and international patents. **SYBR is trademark of Molecular Probes, Inc. and GelStar is trademark of FMC corporation. Please also see our EvaGreenTM(cat#31000), a breakthrough nucleic acid dye ideally suited for quantitative real-time PCR(qPCR). By incorporating a smart "release-on-demand" DNA-binding technology, EvaGreenTM has low PCR inhibition while exhibiting superior sensitivity. Similar to our GelRedTM, EvaGreenTM has remarkable stability. |
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文献和实验a 5 mg/mL DAPI stock solution (14.3 mM for the dihydrochloride or 10.9 mM for the dilactate), dissolve the contents of one vial (10 mg) in 2 mL of deionized water (dH2 O) or dimethylformamide (DMF). The less water-soluble DAPI dihydro-chloride
to stain faster. Alternatively,soak gel instain for 1 hr at room temperature. 3.Pour stain back into bottle for later reuse.Rinse gel with water.Add ~200 ml protein gel destain.Microwave ~45 sec until the solution just starts to boil.Incubate with gentle
from protein to protein. Caution: nucleic acids are stain. Fresh Solutions 0.02% Sodium Thiosulfate (Na2S2O3) 0.1% AgNO3 for minigel and 0.15% for large gel. Stored in 4ºC before use. 2% Na2CO3 + 0.04% formaldehide. Prepare fresh. Note: Wear
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