GelGreenTM Nucleic Acid Gel St

GelGreen^TM is a sensitive, stable and enviornmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen is far more sensitive than SYBR Safe (Figure 1). Unlike SYBR dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally. GelGreen has a UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (Figure 2). Thus, GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader).

GelGreen can be used for either post gel staining or precast gel staining.

A series of safety tests have confirmed that GelGreen^TM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelGreen can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed/GelGreen Safety Report here.

We offer GelGreen^TM 10,000X solution in DMSO and in water (cat#41005) for better safety. We also offer 10mL bulk pack size of GelGreen^TM 10,000X solution in water (cat#41005-1).

FEATURES

Safer than EB Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic Easy disposal Passed environmental safety tests for direct disposal down the drain or in regular trash Ultra-sensitive Much more sensitive than EtBr and SYBR Safe Extremely stable Available in water, stable at room temperature for long-term storage and microwavable

Simple to use Very simple procedures for either precast or post gel staining Perfectly compatible with a standard UV transilluminator or a gel reader with visible light excitation GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 2) Perfectly compatible with downstream applications Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.