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- PVT11580
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pSV40-Fcho1-m (1073-2435bp)
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pSV40-Fcho1-m (1073-2435bp)/ pSV40-Fcho1-m (1073-2435bp)璐ㄧ矑
pSV40-Fcho1-m(1073-2435bp)
Catalog No.PVT11580
Packing 2ug
pSV40-Fcho1-m(1073-2435bp) Alternative name
pSV40-Fcho1-m(1073-2435bp)pSV40-Fcho1-m(1073-2435bp) vector, pSV40-Fcho1-m(1073-2435bp) plasmid, pSV40-Fcho1-m(1073-2435bp) map, pSV40-Fcho1-m(1073-2435bp) sequence,
Promoter -
Replicon -
Terminator -
Resistance Amp
Strain DH5alpha
Culture conditions LB,37degrees centigrade
Forward primer -
Reverse primer -
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文献和实验PLASMID MIDI-PREP FROM BACTERIA
phase to a 1.5 mL tube. 18. Add 40 m L of 3M NaOAc (1/10 vol) and ~1 mL of 100% EtOH (to the top of the tube) and mix by inversion (optional: leave ON) 19. Centrifuge at top speed for 15 mins and aspirate
Adjuvants for Plasmid DNA Vaccines
(2 ). More recently, plasmid DNA expression vectors were improved such that an average of 300 ng of gene product could be produced from single intramuscular (i.m.) injections of plasmid DNA, and up to 40 μg of gene product could be produced after multiple injections
PREPARATION OF PLASMID DNA FOR SEQUENCING
. Incubate at 37℃ overnight with vigorous shaking. Follow the Plasmid Quick Prep protocol through the potassium acetate step to isolate plasmid DNA. Add DNAse-free RNAse to 50µg/ml, incubate, 37℃ from 10-30 minutes. Phenol extract the solution, saving
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