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- PVT11578
- 2025年07月08日
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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pSV40-Nolc1-m
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pSV40-Nolc1-m/ pSV40-Nolc1-m璐ㄧ矑
pSV40-Nolc1-m
Catalog No.PVT11578
Packing 2ug
pSV40-Nolc1-m Alternative name
pSV40-Nolc1-mpSV40-Nolc1-m vector, pSV40-Nolc1-m plasmid, pSV40-Nolc1-m map, pSV40-Nolc1-m sequence,
Promoter -
Replicon -
Terminator -
Resistance Amp
Strain DH5alpha
Culture conditions LB,37degrees centigrade
Forward primer -
Reverse primer -
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
PLASMID MIDI-PREP FROM BACTERIA
phase to a 1.5 mL tube. 18. Add 40 m L of 3M NaOAc (1/10 vol) and ~1 mL of 100% EtOH (to the top of the tube) and mix by inversion (optional: leave ON) 19. Centrifuge at top speed for 15 mins and aspirate
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