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- LSM BIO
- 进口
- PVT10348
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
mKO2-SLBP (18-126)
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
mKO2-SLBP (18-126)/ mKO2-SLBP (18-126)璐ㄧ矑
mKO2-SLBP(18-126)
Catalog No. PVT10348
Packing 2ug
Function ORF expression plasmid
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验A fundamental step in molecular biology is the cloning of a DNA fragment insert into a plasmid vector. This allows the cloned fragment to be replicated upon transformation of the recombinant molecule into a bacterial cell (see Chapters
Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30°C, 230-250rpm (best in 5ml bijou) Spin yeast culture at 13,000rpm for 5 min (microfuge) Pour
Objective: Mini-Prep procedure is used to isolate small plasmid DNA from bacteria while limiting contaminating proteins and genomic DNA. The plasmid quality is acceptable for restriction analysis, sequencing, cloning, or other purposes
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