BstAPI内切酶价格

特别提示：包括BstAPI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：BstAPI内切酶
英文名称：BstAPI
产品货号：GS2187
产品规格：100U

储存条件：冷冻（-20℃）

使用说明

High enzyme concentration may result in star activity.At 37℃ activiti is 50%from maximum.The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl.
Recognition sites	GCANNNN↑NTGC
来源	Bacillus stearothermophilus AP
Assayed on	Lambda DNA
Optimal buffer	W(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature	60℃
Storage conditions	10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;and 50%glycerol Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 60℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	ApaBI^

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