Direct-zol RNA Miniprep Kits（总

HIGHLIGHTS

• Easy Handling: Bypass chloroform, phase separation and precipitation steps.
• NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
• Non-Biased: Complete RNA recovery without miRNA loss.

DESCRIPTION

The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for sub.sequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.