Direct-zol RNA Miniprep Plus K

HIGHLIGHTS

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• Easy Handling: Bypass chloroform, phase separation and precipitation steps.
• NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
• Non-Biased: Complete RNA recovery without miRNA loss.

DESCRIPTION

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The Direct-zol™ RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.

	MiniPrep Plus	MiniPrep	MicroPrep
RNA Recovery	100 µg	50 µg	10 µg
Minimum Elution	50 µl	25 µl	6 µl
(Animal) Cells	≤ 107	≤ 5 x 106	≤ 106
Tissue	≤ 50 mg	≤ 25 mg	≤ 5 mg