Bse8I内切酶价格

特别提示：包括Bse8I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：Bse8I内切酶
英文名称：Bse8I
产品货号：GS2133
产品规格：500U

储存条件：冷冻（-20℃）

使用说明

High enzyme concentration(more than 5-fold overdigestion on 1μgsubstrate)may result in star activity.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl.
Recognition sites	GATNN↑NNATC
来源	Bacillus species 8
Assayed on	Lambda DNA
Optimal buffer	G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)
Optimal temperature	60℃
Storage conditions	10mMTris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme about 80%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	Long incubation not recommended owing to ℃currence of star activity
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	BsaBI,BseJI,BsiBI,BsrBRI,MamI

我公司销售的Bse8I内切酶价格，Bse8I限制性内切酶质量上佳，价格普惠，欢迎广大新老客户踊跃订购。