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- 技术资料
- 库存:
大量
- 英文名:
QuantiNova SYBR Green PCR Kit (500)
- 保质期:
1年
- 供应商:
科邦兴业(北京)科技有限公司
- 保存条件:
-20 ℃保存
- 规格:
kit

凯杰 QIAGEN 208054 QuantiNova SYBR Green PCR Kit (500)
For 500 x 20 µl reactions: 3 x 1.7 ml 2x QuantiNova SYBR Green PCR Master Mix, 500 µl QuantiNova Yellow Template Dilution Buffer, 1 ml QN ROX Reference Dye, 1.9 ml Water
通过基于SYBR Green的qPCR获得好的结果
- 通过新型热启动机制获得良好的特异性
- 通过内置颜色指示剂实现准确的反应构建
- 精确、灵敏的检测低至单拷贝的靶序列
- 采用超快速PCR技术,提高样本通量和反应速度
- 与QuantiTect Primer Assay实现无缝对接
QuantiNova SYBR Green PCR Kit具有高特异性、敏感度、反应速度和处理安全性,为SYBR Green-based qPCR技术树立了全新标准。创新的热启动技术显著增加了real-time PCR特异性,通过使用优化的试剂,能够在任何real-time PCR仪上从cDNA或gDNA——甚至是数量低至1个拷贝的靶标——获得精确的定量检测结果。此外,内置的颜色指示剂帮助确保加样的准确性。
Product Details
Featuring enhanced specificity, sensitivity, speed, and process safety, the QuantiNova SYBR Green PCR Kit (SYBR qPCR kit) sets new standards in SYBR Green-based qPCR. A novel hot-start mechanism significantly increases the specificity of real-time PCR and optimized chemistry produces accurate quantitative results from cDNA or gDNA on any real-time PCR cycler with detection of even a single target copy. Finally, a built-in visual indicator ensures correct pipetting.
Avoid the deleterious effects of mispriming at lower temperatures with the QuantiNova Probe PCR Kit (probe qPCR kit). Based on a novel, antibody-mediated, hot-start mechanism, the kit enhances the specificity and efficiency of probe-based real-time PCR to provide accurate, singleplex or duplex, cDNA or gDNA analysis on various real-time PCR cyclers. The added convenience of extreme stability for up to 100 hours at room temperature, without a need for any cooling agent, makes it ideal for handling of high-throughput samples and automated workflows. An in-built tracking system for visual identification of correct pipetting gives you absolute peace of mind while extremely sensitive detection of even low target amounts ensures reliable qPCR results every time.
QuantiNova Multiplex PCR Kits (multiplex qPCR kits) enable fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote ultrafast multiplex real-time PCR within 1 hour. The combination of a unique hot start and PCR buffer system in the ready-to-use 4x master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization, also providing automated reaction set up options at room temperature. An in-built tracking system for visual identification of correct pipetting helps to prevent human errors, and the combination with QuantiNova Reverse Transcription Kit for cDNA synthesis allows inclusion of QuantiNova Internal Control RNA to monitor successful reverse transcription and qPCR.
Performance
Want to try the QuantiNova SYBR Green PCR Kit for the first time? Request a quote for a trial kit.
Want to try the QuantiNova Probe PCR Kit for the first time? Request a quote for a trial kit.
Want to try QuantiNova Multiplex PCR Kits for the first time? Request a quote for a trial kit
Applications
The QuantiNova SYBR Green PCR Kit can be used for SYBR Green-based gene expression analysis of cDNA targets and quantitative gDNA analysis on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, LIFE Technologies, Roche, and Agilent, as well as the Rotor-Gene Q.
QuantiNova Probe PCR Kits can be used for probe-based gene expression analysis of cDNA targets and quantitative gDNA analysis on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche, and Agilent.
QuantiNova Multiplex PCR Kits can be used for multiplex gene expression analysis of cDNA or gDNA targets on any real-time cycler. To fully exploit the capability of multiplex benefits, we recommend instruments providing up to 5plex capacity, such as the Rotor-Gene Q.
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文献和实验REAL TIME PCR WITH SYBR GREEN I 建议PROTOCOL
1、反应体系 25ulCocktail 20 ul: Primer FP 1 ul + Primer RP 1u + 2 * SYBR GREEN I MIX 12.5 + H2O 5.5 ul cDNA 5 ul2、Primer 浓度,需要摸索,从200nM到700nm等,设置不同浓度。3、每个引物浓度,从well 1到12设置4个样本,阳性cDNA 1和2,NTC以及H2O,做triplicate。4、若用ABI7000或7700,所有的PCR条件可设置成一致的,减少麻烦,当然,引物
以500 rpm的速度旋转,能保证样品间具有极高的温度均一性(±0.01ºC),从而大大提高了反应的稳定性。 可同时检测FAM、JOE、ROX和Cy5等4种荧光信号,适应于多个目标基因的复合检测。 Rotor Gene 3000型荧光定量 PCR仪 的主要参数: Excitation Source: 470
actin,山东大学提供样品基因。cDNA制备:测定小鼠肝脏总RNA浓度后,使用BioTeke Super RT Kit 制备20μl cDNA.操作步骤参照试剂盒说明书。一步法荧光定量PCR:Bioteke One-step SYBR real-time RT-PCR Kit20μl反应体系包括:10μl 2×One-step SYBR premixture,7.2μl RNA Free Water,0.4μl Primer F,0.4μl Primer R,1μl cDNA,1μl One-step











