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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
冰袋运输,2-8℃避光保存6个月。
- 保质期:
6个月
- 库存:
大量
- 英文名:
Picogreen dsDNA Quantitation Reagent
- 供应商:
翌圣生物科技(上海)股份有限公司
- 规格:
100T(12640ES60)/500T(12640ES76)
| 规格: | 100T(12640ES60) | 产品价格: | ¥686.00 |
|---|---|---|---|
| 规格: | 500T(12640ES76) | 产品价格: | ¥2696.00 |
产品组分

| 编号 | 组分名称 | 浓度 | 12640ES60 | 12640ES76 |
| 12640-A | dsDNA Reagent | 200×concentrate in DMSO | 250 μL | 1.25 mL |
| 12640-B | dsDNA Buffer | Not applicable | 50 mL | 250 mL |
| 12640-C | dsDNA Standard 1 | 0 ng/μL in TE buffer | 1 mL | 5×1 mL |
| 12640-D | dsDNA Standard 2 | 10 ng/μL in TE buffer | 1 mL | 5×1 mL |
运输和保存
冰袋运输,2-8℃避光保存12个月。
注意事项
1)荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭;
2)对于DNA标准品,每次使用前先上下颠倒数次后瞬时离心数秒(请勿涡旋振荡);
3)为了您的安全和健康,请穿实验服并戴一次性手套操作。
4)本产品仅用作科研用途!
实验步骤
一、使用Qubit荧光仪进行dsDNA定量检测分析

| 污染物 | 10 µL样品中的浓度 | 样品检测时浓度 | 检测结果 |
| Salts | |||
| Ammonium acetate | 200 mM | 10 mM | OK |
| Sodium acetate | 200 mM | 10 mM | OK |
| Sodium chloride | 200 mM | 10 mM | OK |
| Magnesium chloride | 40 mM | 2 mM | OK |
| Organic Solvents | |||
| Phenol | 2% | 0.1% | OK |
| Ethanol | 20% | 1% | OK |
| Chloroform | 4% | 0.2% | OK |
| Detergents | |||
| Sodium dodecyl sulfate | 0.2% | 0.01% | OK |
| Triton X-100 | 0.02% | 0.001% | OK |
| Other Compounds | |||
| Bovine serum albumin | 400 μg/mL | 20 μg/mL | OK |
| RNA | 1×* | 1×* | OK |
| dNTPs | 2 mM | 100 μM | OK |
| Polyethylene glycol | 20% | 1% | OK |
| Agarose | 2% | 0.1% | OK |
相关产品
| 建库试剂盒 | 产品编号 | 规格 |
| Hieff NGSTM MaxUP II DNA Library Prep Kit for Illumina® | 12200ES08 | 8 libraries |
| 12200ES24 | 24 libraries | |
| 12200ES96 | 96 libraries | |
| Hieff NGSTM MaxUp Dual-mode mRNA Library Prep Kit for Illumina® | 12301ES08 | 8 libraries |
| 12301ES24 | 24 libraries | |
| 12301ES96 | 96 libraries | |
| 文库定量 | 产品编号 | 规格 |
| Hieff NGSTM Library Quantification Kit for Illumina®, qPCR Master Mix | 12302ES05 | 500 T |
| Hieff NGSTM Library Quantification Kit for Illumina®, DNA Standard 1-6 | 12307ES09 | 6×96 μL |
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- 作者
- 内容
- 询问日期
文献和实验[1] Duan XZ, Sun JT, Wang LT, et al. Recent infection by Wolbachia alters microbial communities in wild Laodelphax striatellus populations. Microbiome. 2020;8(1):104. Published 2020 Jul 2. doi:10.1186/s40168-020-00878-x(IF:11.607)
[2] Zhang Y, An C, Zhang Y, et al. Microfluidic-templating alginate microgels crosslinked by different metal ions as engineered microenvironment to regulate stem cell behavior for osteogenesis. Mater Sci Eng C Mater Biol Appl. 2021;131:112497. doi:10.1016/j.msec.2021.112497(IF:7.328)
[3] An C, Liu W, Zhang Y, et al. Continuous microfluidic encapsulation of single mesenchymal stem cells using alginate microgels as injectable fillers for bone regeneration. Acta Biomater. 2020;111:181-196. doi:10.1016/j.actbio.2020.05.024(IF:7.242)
[4] An C, Zhang Y, Li H, et al. Thermo-responsive fluorinated surfactant for on-demand demulsification of microfluidic droplets. Lab Chip. 2021;21(18):3412-3419. Published 2021 Sep 14. doi:10.1039/d1lc00450f(IF:6.799)
[5] Gu L, Ren F, Fang X, Yuan L, Liu G, Wang S. Exosomal MicroRNA-181a Derived From Mesenchymal Stem Cells Improves Gut Microbiota Composition, Barrier Function, and Inflammatory Status in an Experimental Colitis Model. Front Med (Lausanne). 2021;8:660614. Published 2021 Jun 24. doi:10.3389/fmed.2021.660614(IF:5.093)
[6] Zhao H, Tang X, Wu M, et al. Transcriptome Characterization of Short Distance Transport Stress in Beef Cattle Blood. Front Genet. 2021;12:616388. Published 2021 Feb 10. doi:10.3389/fgene.2021.616388(IF:4.599)
[7] Gao ZR, Liu Q, Zhao J, et al. A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. PLoS Negl Trop Dis. 2022;16(5):e0010379. Published 2022 May 2. doi:10.1371/journal.pntd.0010379(IF:4.411)
[8] Li J, Li X, Li M, et al. Differential early diagnosis of benign versus malignant lung cancer using systematic pathway flux analysis of peripheral blood leukocytes. Sci Rep. 2022;12(1):5070. Published 2022 Mar 24. doi:10.1038/s41598-022-08890-x(IF:4.380)
[9] Liang H, Zhang X, Ma Z, et al. Association of CYP3A5 Gene Polymorphisms and Amlodipine-Induced Peripheral Edema in Chinese Han Patients with Essential Hypertension. Pharmgenomics Pers Med. 2021;14:189-197. Published 2021 Feb 2. doi:10.2147/PGPM.S291277(IF:3.912)
[10] Bing XL, Zhao DS, Peng CW, Huang HJ, Hong XY. Similarities and spatial variations of bacterial and fungal communities in field rice planthopper (Hemiptera: Delphacidae) populations. Insect Sci. 2020;27(5):947-963. doi:10.1111/1744-7917.12782(IF:2.791)
[11] Zhang Y, Dai J, Yan L, Sun Y. Intra-articular injection of decellularized extracellular matrices in the treatment of osteoarthritis in rabbits. PeerJ. 2020;8:e8972. Published 2020 Apr 22. doi:10.7717/peerj.8972(IF:2.379)
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