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- 详细信息
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 器官来源：
皮肤
- 生长状态：
贴壁生长
- 相关疾病：
黑色素瘤
- ATCC Number：
CRL-1676™
- 年限：
58 years
- 库存：
大量
- 运输方式：
冻存运输
| Designations: | WM-266-4 | ||
| Depositors: | M Herlyn | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: skin Disease: melanoma |
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| Cellular Products: | proteoglycan antigen characteristic of melanoma | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 12,13 D16S539: 11,12 D5S818: 13 D7S820: 8 THO1: 7,9 TPOX: 8,11 vWA: 15,17 |
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| Cytogenetic Analysis: | The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell. | ||
| Age: | 58 years | ||
| Gender: | female | ||
| Comments: | The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma. Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675 ). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 derived from same individual:ATCC CRL-1675 |
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WM-266-4
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