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- 2026年01月18日
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- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 器官来源：
皮肤
- 生长状态：
贴壁生长
- 相关疾病：
黑色素瘤
- ATCC Number：
CRL-1676™
- 年限：
58 years
- 库存：
大量
- 运输方式：
冻存运输
| Designations: | WM-266-4 | ||
| Depositors: | M Herlyn | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: skin Disease: melanoma |
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| Cellular Products: | proteoglycan antigen characteristic of melanoma | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 12,13 D16S539: 11,12 D5S818: 13 D7S820: 8 THO1: 7,9 TPOX: 8,11 vWA: 15,17 |
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| Cytogenetic Analysis: | The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell. | ||
| Age: | 58 years | ||
| Gender: | female | ||
| Comments: | The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma. Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675 ). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 derived from same individual:ATCC CRL-1675 |
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文献和实验Morphological Assessments of Neonatal HypoxiaIschemia: White Matter and Blood-Brain Barrier Injury
Damages to the blood–brain barrier (BBB) and white matter (WM)/oligodendrocytes (OLs) are typical pathological findings in infants or animal models of neonatal hypoxia–ischemia (NHI). These injuries in turn produce severe neurological
有效抑制肿瘤术后复发！浙大顾臻等开发神奇水凝胶，实现缓释型免疫疗法
胶中的 CAR-T 细胞和血小板的释放，结果表明 CAR-T 细胞和血小板都是持续释放的。96 小时后，几乎所有的血小板都被释放，而 CAR-T 细胞的释放只有 50%。血小板的快速释放可能是由于它们在水凝胶内明显的运动，血小板的平均迁移距离是 CAR-T 细胞的四倍。 接下来，他们使用体外共培养实验评估了水凝胶内 CAR-T 细胞的抗肿瘤活性。结果发现，72h 后，WM115 黑色素瘤细胞和 T 细胞的百分比在 T@gel 和 T-platelet@gel 两种水凝胶中均无显著变化。相比之下，在水凝
(SDS-PAGE), as shown in Fig. 1. Fig. 1. 2-DE gel pattern of WM35 (melanoma) cell line.
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