Methods in Functional Proteomics: Two-Dimensional Polyacrylamide Gel Electrophoresis With Immobilized pH Gradients, In-Gel Digestion, and Identificati
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The recent combination of several technology platforms has revolutionized the analysis of expressed cellular proteins (proteomics). Large-scale separation and visualization of proteins is accomplished using two-dimensional gel electrophoresis (2-DE). Proteins are separated first by isoelectric focusing (IEF) using immobilized pH gradient (IPG) strips. Following charge separation, proteins are further resolved by molecular mass using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), as shown in Fig. 1.
Fig. 1. 2-DE gel pattern of WM35 (melanoma) cell line.
