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- 2025年09月11日
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- 详细信息
- 文献和实验
- 技术资料
- 器官来源：
骨髓
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 库存：
大量
- 相关疾病：
正常
- ATCC Number：
CRL-12103™
- 生长状态：
悬浮生长
- 品系：
DBA/2
| Designations: | FDC-P1 | ||
| Depositors: | University of Virginia Patent Foundation | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Organ: bone marrow Strain: DBA/2 Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Antigen Expression: | Thy-1.2 (24%) | ||
| Cytogenetic Analysis: | diploid | ||
| Comments: | The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68 ). They are dependent upton WEHI-3 conditioned medium (WEHI-3CM) for continued growth. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 65%; fetal bovine serum, 10% supplemented with 25% mouse Interleukin-3 culture supplement. (Supplement purchased from BD Biosciences, Catalog No. 354040). Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 23429: Dexter TM, et al. Growth of factor-dependent hemopoietic precursor cell lines. J. Exp. Med. 152: 1036-1047, 1980. PubMed: 6968334 | ||
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文献和实验头孢菌素 P1 是头孢菌产生的抗菌物质。通常认为 结构与梭链孢酸很相似，作用机理也类似。
Characterization of P1 (Adenosine) Purinoceptors
Abstract Table of Contents Materials Figures Literature Cited Abstract The purine nucleoside
Assembling New Escherichia coli Strains by Transduction Using Phage P1
A protocol is described that allows the transfer of genetic material from one Escherichia coli strain to another using bacteriophage P1. P1 transduction can be used to construct new bacterial strains containing multiple alleles, to restore
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