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- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- ATCC Number：
CRL-1605™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 生长状态：
悬浮生长
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 器官来源：
脾
| Designations: | HFN 36.3 | ||
| Depositors: | RC Schoen, KL Bentley, RJ Klebe | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Organ: spleen Cell Type: hybridoma: B lymphocyte; |
||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human fibronectin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | H-2d | ||
| Comments: | The antibody cross-reacts with fibronectins from 26 species including 15 primates. Mice were immunized with purified human fibronectin. Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). Formerly ATCC HB-40. |
||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; newborn bovine calf serum, 10% |
||
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml. |
||
| References: | 57999: Schoen RC, et al. Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125 | ||
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文献和实验CD36 分子 CD36 常用单克隆抗体或代号： 5F1,ESIVC7,OKM5 主要表达细胞： Pt,M,Mac, (B) [Pt] 分子质量(kDa)和结构： gp88,血小板GPⅣ 功 能： 结合ECM(CA、Thr),血小板粘附 CD36 Aka platelet GPIV or GPIIIb; thrombospondin receptor Scavenger
36 Large-Scale Extraction of Proteins
Purification of proteins using aqueous two-phase systems (ATPS) has been successfully carried out on large scale for more than a decade (1 –17 ). One of the major advantages of ATPS is that the scaling up is possible through the use
人可溶性CD36 分子 ( sCD36 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 sCD36 单抗包被于酶标板上，标准品和样品中的 sCD36与单抗结合，加入生物素化的抗人 sCD36 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝
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