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- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
成纤维细胞
- 相关疾病：
正常
- ATCC Number：
CCL-153™
- 细胞形态：
成纤维样
- 库存：
大量
- 运输方式：
冻存运输
- 年限：
fetus
- 器官来源：
肺
- 生长状态：
贴壁生长
| Designations: | HFL1 | ||
| Depositors: | RG Crystal | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
||
| Source: | Organ: lung Disease: normal Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 9,11 D5S818: 12 D7S820: 9,10 THO1: 7,9 TPOX: 6,9 vWA: 17 |
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| Cytogenetic Analysis: | normal human; diploid; stable | ||
| Isoenzymes: | G6PD, B | ||
| Age: | fetus | ||
| Ethnicity: | Caucasian | ||
| Comments: | The cells have been observed to attain at least a Population Doubling Level (PDL) of 31 without obvious signs of senescence. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020 |
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| References: | 23400: Breul SD, et al. Control of collagen production by human diploid lung fibroblasts. J. Biol. Chem. 255: 5250-5260, 1980. PubMed: 7372635 | ||
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文献和实验相关实验
）的整张培养支架，所有培养支架及培养板均经过γ射线辐照后包装，以确保无菌，使用XanoMatrix™培养支架进行细胞接种和培养板孔中RNA/DNA//蛋白质的提取等研究手段与现时技术指导一致。培养支架连同培养的细胞还可以轻松地用镊子移除，以作进一步处理。 产品技术总览应用癌症研究、干细胞/再生医疗、组织塑形、共培养、药物毒性筛选等。 现用细胞类型癌症细胞株（MDA-MB-231、MCF-7、A549）；成纤维细胞（NIH/3T3、HFL1）；内皮细胞（HUVEC、EAhy926）；干细胞（人和小鼠
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HFL1
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