- 询价
- 2026年02月09日
15 年金牌会员
企业认证
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 组织来源：
cervix
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 年限：
31
- 库存：
大量
- 运输方式：
冻存运输
- 相关疾病：
腺癌
- ATCC Number：
CRL-2972™
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
| Designations: | HeLaRC32 [HeRC32] | ||
| Depositors: | P Moullier | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Tissue: cervix Disease: adenocarcinoma |
||
| Cellular Products: | Rep proteins (Rep 78, Rep 68, Rep 52 and Rep 40) Cap proteins (VP1, VP2 and VP3) |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | These cells generate rAAV preparations with high titers of infectious particles which are essentially free of adenoviruses for biological, preclinical, clinical or pharmaceutical applications. HelaRC32 is a stable packaging cell line expressing the rep and cap genes for recombinant adeno-associated virus type 2 (rAAV-2) assembly which constitutes an attractive alternative to transient transfection protocols. [PubMed: 11119606]. |
||
| DNA Profile (STR): | D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 THO1: 7 TPOX: 8, 12 CSF1PO: 9, 10 Amelogenin: X |
||
| Age: | 31 | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| Comments: | HelaRC32 is a stable packaging cell line expressing the rep and cap genes for recombinant adeno-associated virus type 2 (rAAV-2) assembly which constitutes an attractive alternative to transient transfection protocols. [PubMed: 11119606]. This is a clone of HeLa cells that harbors one to two rep-cap gene copies per cell. Upon vector transfection and adenovirus infection, efficient rAAV assembly correlated with a 100-fold amplification of the integrated rep-cap sequence with the inverted terminal repeats (ITRs) deleted. These cells generate rAAV preparations with high titers of infectious particles which are essentially free of adenoviruses for biological, preclinical, clinical or pharmaceutical applications. [PubMed: 10953917]. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
||
| Subculturing: | Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
|
||
| Preservation: | Freeze medium: Dulbecco's Modified Eagle's Medium, 70%; fetal bovine serum, 20%; DMSO, 10% | ||
| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002 Recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Phosphate-buffered saline: ATCC 30-2200 Cell culture tested DMSO: ATCC 4-X Erythrosin B vital stain solution: ATCC 30-2404 Recommended adenovirus: ATCC VR-1616, rAAV2 Recommended vector: ATCC MBA-331, pTR-UF- |
||
| References: | 16172640: Chadeuf G, et al. Efficient recombinant adeno-associated virus production by a stable rep-cap HeLa cell line correlates with adenovirus-induced amplification of the integrated rep-cap genome. J. Gene Med. 2(4):260-268, 2000. PubMed: 10953917. 16172684: Tessier J, et al. Characterization of adenovirus-induced inverted terminal repeat-independent amplification of integrated adeno-associated virus rep-cap sequences. J. Virol. 75(1):375-383, 2001. PubMed: 11119606 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验相关实验
人 白细胞介素 - 32（ IL- 32） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 白细胞介素32（IL-32）的 含量。 实验原理 ： 本试剂盒应用 双抗体 夹心法测定 标本 中 人白细胞介素 - 32（ IL- 32） 水平。用纯化的 人白细胞介素 - 32（ IL- 32）抗体 包被微孔板，制成固相 抗 体，往包被 单抗
人白细胞介素32γ(IL-32γ)酶联免疫分析（ELISA）
人 白细胞介素32γ(IL-32γ) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 白细胞介素32γ(IL-32γ)的 含量。 实验原理 ： 本试剂盒应用 双抗体 夹心法测定 标本 中 人白细胞介素32 γ (IL-32 γ ) 水平。用纯化的 人白细胞介素32 γ (IL-32 γ ) 抗体 包被微孔板，制成固相 抗 体，往包被 单抗
放射性同位素 32 P 以 14.3 日的半衰期放出β射线而衰变。遗传物质 DNA 是以磷酸二酯键将核苷酸连接起来的多核苷酸链。例如，将 32 P 标记到 DNA 上，再将这种 DNA 掺入噬菌体或细菌，储存起来，测定其生存率，就可观察到其生存率按 DNA 分子的大小和 32 P 的活性，随时间而减少。最后，随着 DNA 中 32P 的衰变而导致 DNA 链的断裂（单链或双链）， DNA 失去生物活性，称为 32 P 衰变失活。
技术资料暂无技术资料 索取技术资料
HeLaRC32 [HeRC32]
询价
