- 询价
- 2026年02月09日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 库存：
大量
- 器官来源：
宫颈
- 相关疾病：
腺癌
- ATCC Number：
CRL-13011™
- 年限：
31 years
| Designations: | HeLa NR1 | ||
| Depositors: | United States Government - DHHS | ||
| Biosafety Level: | 2 [cells contain papova and SV40 viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: cervix Disease: adenocarcinoma Cell Type: HeLa cells transfected with plasmid pSV2neoNR101 |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Reverse Transcript: | Y | ||
| Age: | 31 years | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| HeLa Markers: | Y | ||
| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.5 mg/ml G418 and 10% fetal bovine serum Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: 1:6 to 1:10 Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-12510 |
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| References: | 61432: Parker JE, Kiel JL. Nitrate reductase-transfected HeLa cells for cancer and microwave bioeffects research. US Patent 6,326,196 dated Dec 4 2001 | ||
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文献和实验N -methyl-d -aspartate receptors (NMDARs) are ligand-gated ion channels belonging to the family of ionotropic glutamate receptors. Functional NMDARs are heterotetrameric assemblies of NR1 subunits with at least one type of NR2 subunits
HeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studies of the cell cycle
Preparation of HeLa Nuclear Extracts
Promoter-specific in vitro transcription by RNA polymerase II is now achievable with cell extracts prepared from a variety of animal and plant sources (1 –3 ). For extracts prepared from mammalian cells, HeLa cells provide many advantages
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