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- 文献和实验
- 技术资料
- 年限：
stage 4
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 器官来源：
肺
- 细胞形态：
上皮样
- 库存：
大量
- 相关疾病：
腺癌
- ATCC Number：
CRL-5911™
- 运输方式：
冻存运输
| Designations: | NCI-H2009 [H2009] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Tumor Stage: stage 4 Disease: adenocarcinoma Derived from metastatic site: lymph node |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 12 D5S818: 13 D7S820: 9,11 THO1: 9.3 TPOX: 8 vWA: 14 |
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| Age: | 68 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was established in August 1988. The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 30 pack years. A lymphoblastoid line from the same patient is available as ATCC CRL-5961 . |
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| Propagation: | ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum
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| Preservation: | 85% Complete Growth Medium + 10% FBS + 5% DMSO. | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 normal (or near-normal) cell line established from the same patient:ATCC CRL-5961 |
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| References: | 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. | ||
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文献和实验NCI-H526来源于一名55岁白人男性小细胞肺癌（Stage E）患者的骨转移灶，从肺部分离获得。该细胞高表达两个小细胞肺癌生化标志物——神经元特异性烯醇化酶和脑肌酸激酶同工酶，是肺癌研究中常用的体外模型之一。 形态特征与生长方式 悬浮生长，细胞以大量聚团形式存在。刚复苏时细胞是分散的，24-48小时后状态逐渐恢复，开始聚团生长。传代时不需要完全吹散成单细胞，把大团吹成小团即可，避免团块中间的细胞缺乏营养。 培养要点与注意事项 培养基选用1640添加10%胎牛血清及1%双抗
都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
人胃癌细胞(未分化) SNL-105 KATO III 人胃癌细胞 SNL-106 NCI-N87 人胃癌细胞 SNL-107 SNU-1 人胃癌细胞 SNL-173 MKN45 人低分化胃癌细胞 SNL-176 MKN74 人胃癌细胞 SNL-304 GES-1 人胃粘膜细胞 SNL-325 Hs-746T 人胃癌细胞 SNL-365 MFC 小鼠胃癌细胞 SNL
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