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- 文献和实验
- 技术资料
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- ATCC Number：
HB-131™
- 生长状态：
悬浮生长
- 库存：
大量
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | I(TL.m9) | ||
| Depositors: | F Shen | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against TL antigen | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with ASLI (a leukemia cell derived from A mice). Spleen cells were fused with NS-1 mouse myeloma cells. The antibody reacts with either TL.5 or TL.6 or both Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 988: Shen FW, et al. Further polymorphism of the Tla locus defined by monoclonal TL antibodies. Immunogenetics 15: 573-578, 1982. PubMed: 7106865 | ||
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文献和实验M9 Plate Supplementation Table
M9 Plate Supplementation Table Amino Acid Stock Amount To Spread (per 25 ml plate) URA 1 mg/ml 280 µl LEU
Expression Protocol in M9 Minimal Media via T7 Promoter
The following protocol has been used successfully to 15N or 13C/15N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 minimal media begins with preparing a 5x stock solution of M9 salts. Generally, M9 salts contain
assays for detection and characterization of modulators of SK channel activity. In the present chapter, we describe two fluorescence-based Tl+ influx assays for detection of positive and negative SK channel modulators.
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