HCE-2 [50.B1]产品图

HCE-2 [50.B1]

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  • 2026年11月13日
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 组织来源:

      cornea

    • 生长状态:

      贴壁生长

    • 细胞形态:

      上皮样

    • 运输方式:

      冻存运输

    • 库存:

      大量

    • 器官来源:

      眼

    • 物种来源:

      人

    • 是否是肿瘤细胞:

      0

    • ATCC Number:

      CRL-11135™

    • 细胞类型:

      其他细胞类型

    Designations: HCE-2 [50.B1]
    Depositors:  CR Kahn, Gillette Medical Evaluation Laboratories
    Biosafety Level: 2 [Cells contain Adeno-12/SV-40 viral sequences ]
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology: epithelial

    Source: Organ: eye
    Tissue: cornea
    Cell Type: epithelialAdenovirus 12-SV40 hybrid transformed
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    DNA Profile (STR): Amelogenin: X,Y
    CSF1PO: 10,13
    D13S317: 8,11
    D16S539: 9,11
    D5S818: 11,12
    D7S820: 8,10
    THO1: 6,9
    TPOX: 10,11
    vWA: 15,18
    Cytogenetic Analysis: aneuploid; Y chromosome is present
    Isoenzymes: AK-1, 1
    ES-D, 1
    G6PD, A
    GLO-I, 2
    Me-2, 1-2
    PGM1, 1
    PGM3, 2
    Gender: male
    Ethnicity: Black
    Comments: A primary culture of normal corneal epithelium was immortalized with Ad12-SV40 hybrid virus by overnight incubation with virus at 37C
    Keratinocyte Serum-Free medium is available from GIBCO, Grand Island, New York.
    Propagation: ATCC complete growth medium: Keratinocyte-Serum Free Medium (Gibco 17005-042)
    Supplemented with frozen additives included (from GIBCO):
    1) 0.05 mg/ml bovine pituitary extract (BPE)
    2) 5 ng/ml epidermal growth factor (EGF).
    NOTE: Do not filter EGF
    And also supplemented with 500 ng/ml hydrocortisone and 0.005mg/ml insulin (not included).
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin-0.53mM EDTA solution.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. (Or neutralize with medium containing 10% fetal bovine serum).
    5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.
    6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Seed cells on flasks precoated with a mixture of 0.01 mg/ml fibronectin, 0.03 mg/ml bovine collagen type I and 0.01 mg/ml bovine serum albumin.
    7. Incubate cultures at 37�C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
    Medium Renewal: Twice per week
    Preservation: Freeze medium: 85% Growth Medium, 10% FBS, 5% DMSO
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time: 30 hrs
    References: 34075: Kahn CR, Rhim J. Human corneal epithelial cell lines with extended lifespan. US Patent 5,585,265 dated Dec 17 1996
    34094: Walker TL, Kahn CR. Human corneal epithelial cell lines with extended lifespan. US Patent 5,672,498 dated Sep 30 1997

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