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- 询价记录
- 文献和实验
- 技术资料
- 组织来源:
cornea
- 生长状态:
贴壁生长
- 细胞形态:
上皮样
- 运输方式:
冻存运输
- 库存:
大量
- 器官来源:
眼
- 物种来源:
人
- 是否是肿瘤细胞:
0
- ATCC Number:
CRL-11135™
- 细胞类型:
其他细胞类型
| Designations: | HCE-2 [50.B1] | ||
| Depositors: | CR Kahn, Gillette Medical Evaluation Laboratories | ||
| Biosafety Level: | 2 [Cells contain Adeno-12/SV-40 viral sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: eye Tissue: cornea Cell Type: epithelialAdenovirus 12-SV40 hybrid transformed |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,13 D13S317: 8,11 D16S539: 9,11 D5S818: 11,12 D7S820: 8,10 THO1: 6,9 TPOX: 10,11 vWA: 15,18 |
||
| Cytogenetic Analysis: | aneuploid; Y chromosome is present | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, A GLO-I, 2 Me-2, 1-2 PGM1, 1 PGM3, 2 |
||
| Gender: | male | ||
| Ethnicity: | Black | ||
| Comments: | A primary culture of normal corneal epithelium was immortalized with Ad12-SV40 hybrid virus by overnight incubation with virus at 37C Keratinocyte Serum-Free medium is available from GIBCO, Grand Island, New York. |
||
| Propagation: | ATCC complete growth medium: Keratinocyte-Serum Free Medium (Gibco 17005-042) Supplemented with frozen additives included (from GIBCO): 1) 0.05 mg/ml bovine pituitary extract (BPE) 2) 5 ng/ml epidermal growth factor (EGF). NOTE: Do not filter EGF And also supplemented with 500 ng/ml hydrocortisone and 0.005mg/ml insulin (not included). Temperature: 37.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: Twice per week |
||
| Preservation: | Freeze medium: 85% Growth Medium, 10% FBS, 5% DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Doubling Time: | 30 hrs | ||
| References: | 34075: Kahn CR, Rhim J. Human corneal epithelial cell lines with extended lifespan. US Patent 5,585,265 dated Dec 17 1996 34094: Walker TL, Kahn CR. Human corneal epithelial cell lines with extended lifespan. US Patent 5,672,498 dated Sep 30 1997 |
||
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文献和实验B是 Backcross,即回交之意,而 B1 则为表示回交第一代的记号。
Science:延长小鼠寿命 50%!提升 VEGF 水平可
导读血管系统是机体所有器官共享的最大的细胞网络,体内的所有细胞都依赖于血管(Blood Vessels, BVs)提供氧气和其他营养物质,还要借助其清除废物。与其他器官一样,随着衰老,血管也会经历与功能下降相关的生物学过程。因此,与年龄相关的血管功能的丧失很可能会影响其他器官的生物学功能,从而引发「血管衰老论」,即血管老化是衰老的主要驱动因素。尽管有类似的假说,但实验证据仍然有限。「血管衰老论」主要基于血管功能的受损可能会扰乱器官内稳态,从而导致与年龄相关的衰弱和疾病的发生,包括动脉硬化、动脉
B1 细胞 B1细胞为CD5+B细胞,所介导的免疫应答特点为:不发生体细胞突变,无亲和力成熟,仅产生低亲和力的IgM抗体,不产生记忆细胞。 1.产生天然IgM 天然IgM是预成抗体的主要成分,主要由D1细胞分泌。这种抗体是多反应性的,能与许多病原体相关糖类抗原结合。如前所述,天然抗体可以看作是一种模式识别分子,产生机制尚未完全阐明,估计和机体在个体发育中遭受过病原体感染有关。天然抗体能保护机体防御细菌(如肺炎链球菌)感染
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