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- 2025年09月23日
企业认证
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- 物种来源：
人
- 是否是肿瘤细胞：
0
- 器官来源：
外周血
- ATCC Number：
CRL-2339™
- 细胞类型：
B淋巴细胞
- 年限：
61 years adult
- 生长状态：
悬浮生长，多细胞聚集
- 细胞形态：
淋巴样
- 库存：
大量
- 运输方式：
冻存运输
| Designations: | HCC1954 BL | ||
| Depositors: | AF Gazdar, AK Virmani | ||
| Biosafety Level: | 2 [Cells contain Herpesvirus; Epstein Barr Virus (EBV) ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Organ: peripheral blood Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935. |
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| Applications: | The cell line was derived from a 61 year-old East Indian female with stage IIA, grade 3, primary ductal carcinoma of the breast. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 8,9 D16S539: 9,11 D5S818: 11,12 D7S820: 10,11 THO1: 6,7 TPOX: 8,9 vWA: 18,19 |
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| Age: | 61 years adult | ||
| Gender: | female | ||
| Ethnicity: | East Indian | ||
| Comments: | HCC1954 BL is a B lymphoblastoid cell line initiated from peripheral blood lymphocytes from the same patient as HCC1954 (ductal carcinoma of the breast) by transformation with Epstein-Barr virus (EBV). The cell line was derived from a 61 year-old East Indian female with stage IIA, grade 3, primary ductal carcinoma of the breast. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 tumor cell line established from the same patient:ATCC CRL-2338 purified DNA:ATCC CRL-2339D purified RNA:ATCC CRL-2339R |
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| References: | 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 | ||
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文献和实验The Nitroreductase/CB1954 Enzyme-Prodrug System
The prodrug CB1954 (5-[aziridin-1-yl]-2,4-dinitrobenzamide) is a weak monofunctional alkylating agent originally synthesized at the Chester Beatty Laboratories in the late 1960s (1 ). The antitumor activity of CB1954 was determined
Direct DNA Injection (p53) into HCC Tumors
Liver tumors, specially HCC, are among the most common malignancies in the world, and their annual world incidence is about 250,000 cases, with a male to female ratio 4:1 (1 ). HCC is one of the most important neoplasms in tropical
1、 首先安装 BL-420F 系统应用软件，安装完成后，驱动程序被复制到硬盘上，注意，你在光盘上是找不到驱动程序的。 安装应用软件后设备驱动程序在硬盘上的位置 2、 如果是原来已经安装驱动程序，但是驱动程序安装有问题，此时你需要更新驱动程序，更新的方法是：在「我的电脑」上单击右键弹出快捷菜单，选择属性，将弹出系统属性对话框，参见下图。 系统属性对话框 3、 在「系统属性」对话框中选择顶部的「硬件」栏，然后单击「设备管理器」按钮弹出「设备管理器」对话
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