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- ATCC Number：
CRL-2079™
- 生长状态：
贴壁生长
- 组织来源：
bronchus
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 年限：
60 years
- 细胞形态：
上皮样
- 库存：
大量
- 器官来源：
肺
| Designations: | HBE4-E6/E7-C1 [NBE4-E6/E7-C1] | ||
| Depositors: | J Viallet | ||
| Biosafety Level: | 2 [Cells contain human papilloma viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: lung Tissue: bronchus Cell Type: epithelial; human papillomavirus 16 (HPV-16) E6/E7 transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Cytogenetic Analysis: | 45, X, -Y, dup (5), -8, +9, -14, -15, -20, -21, -22, +mar1, +mar2, +der(8q;13q) | ||
| Age: | 60 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The HBE4-E6/E7-C1 (formerly NBE4-E6/E7-C1) cell line was cloned from passage 22 of HBE4-E6/E7 (see ATCC CRL-2078) by limiting dilution. The cloned line is more sensitive than the parental line to induction of terminal differentiation by phorbol esters. The cells are non-viable in DMSO and should be frozen in culture medium with 10% glycerol. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture before the cells become confluent. |
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| Preservation: | culture medium, 90%; glycerol, 10% | ||
| Doubling Time: | 24 hrs | ||
| References: | 22447: Viallet J, et al. Characterization of human bronchial epithelial cells immortalized by the E6 and E7 genes of human papillomavirus type 16. Exp. Cell Res. 212: 36-41, 1994. PubMed: 8174640 | ||
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文献和实验HPV 并不是宫颈癌唯一的「元凶」？这种微生物化身「帮凶」，共同促进癌症发生
。引人注目的是，在被 HPV E6E7 上调的基因中，28% 的基因在与沙眼衣原体共感染时恢复到与对照相似的表达水平，或者被强烈下调。 其中，E2F 家族成员是最显著的反向调节转录因子。E2F 靶基因被 HPV E6E7 显著上调，但在沙眼衣原体感染后下调。目前尚不清楚这种沙眼衣原体介导的对 HPV E6E7 诱导的 E2F 靶标的抑制将对宿主细胞和病原体产生什么影响。 为了研究两种病原体对 Rb-E2F1 途径的调节，研究人员用沙眼衣原体感染了 hCEcto 类器官和 hCEcto E6E7
profile曲线上的峰倒过来了。 to be continue ...更多有关el-pro相关的文件，包括操作指导powerpoint演示，屏幕录像演示，还有其他一些可能有用的文件。q请见下一期 感谢战友：hbchendl；原文来源于丁香园论坛：http://www.dxy.cn/bbs/topic/14545324?keywords=%E6%95%AC%E8%AF%B7%E5%85%B3%E6%B3%A8%E7%94%B5%E6%B3%B3%E5%9B%BE%E7
A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
, are RARB2 -transfectants that were established in our laboratory (21 ). MM-1 was also established in our laboratory (6 ). NCI-H23, NCI-H82, NCI-H125, NCI-H157, NCI-H520, and NCI-H596 were supplied by Dr. Adi Gazdar (NCI, NIH, Bethesda, MD). NBE-E6 E7 (22
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