- 询价
- 2026年02月12日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 技术资料
- 库存：
大量
- ATCC Number：
CRL-2741™
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 组织来源：
bronchus
- 运输方式：
冻存运输
- 器官来源：
肺
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 年限：
54 years adult
| Designations: | HBE135-E6E7 | ||
| Depositors: | M Tsao | ||
| Biosafety Level: | 2 [Cells contain HPV-16 E6/E7 viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
||
| Source: | Organ: lung Tissue: bronchus Cell Type: epithelialHPV-16 E6/E7 transformed |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1994 | ||
| Age: | 54 years adult | ||
| Gender: | male | ||
| Comments: | The HBE135-E6E7 cell line was derived from normal bronchial epithelium taken from a man undergoing lobectomy for squamous cell carcinoma [PubMed: 8601403]. Cells from the primary explant in their first passage were infected with the recombinant retrovirus LXSN16E6E7 containing the human papilloma virus (HPV) E6E7 gene. Cells were selected in the presence of 0.4 mg/ml G418 [PubMed: 8601403]. Northern blot hybridization shows that immortalized HBE cells express high levels of mRNA for epidermal growth factor receptor (EGFR), transforming growth factor-alpha (TGF-alpha), and amphiregulin (AR), but not epidermal growth factor (EGF) [PubMed: 8601403]. This cell line may be used as a reference in cDNA microarray studies to demonstrate lung cancer profiles correlating with clinical outcome or biology of tumors [PubMed: 12036904]. | ||
| Propagation: | ATCC complete growth medium: Keratinocyte-Serum Free medium with 5 ng/ml human recombinant EGF (do not filter) and 0.05 mg/ml bovine pituitary extract (Invitrogen, formerly GIBCO-BRL, Cat. No. 17005-042) and supplemented with 0.005 mg/ml insulin and 500 ng/ml hydrocortisone. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: 1:3 to 1:4 Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Doubling Time: | 24 to 30 | ||
| References: | 57773: Tsao MS, et al. Autocrine growth loop of the epidermal growth factor receptor in normal and immortalized human bronchial epithelial cells. Exp. Cell Res. 223: 268-273, 1996. PubMed: 8601403 89587: Wigle DA, et al. Molecular profiling of non-small cell lung cancer and correlation with disease-free survival. Cancer Res. 62: 3005-3008, 2002. PubMed: 12036904 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
HBE135-E6E7
询价
