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- ATCC Number：
CRL-2526™
- 细胞形态：
成纤维样
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 库存：
大量
- 器官来源：
大脑
- 组织来源：
hippocampus
- 品系：
Holtzman
- 物种来源：
褐鼠
- 是否是肿瘤细胞：
0
- 年限：
embryo; 17 day gestation
| Designations: | H19-7/IGF-IR | ||
| Depositors: | R Baserga, A Morrione | ||
| Biosafety Level: | 2 [Cells contain SV40 DNA Viral Sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus deposited as rat | ||
| Morphology: | fibroblast |
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| Source: | Organ: brain Strain: Holtzman Tissue: hippocampus |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | human type I insulin-like growth factor receptor (IGF-IR) | ||
| Age: | embryo; 17 day gestation | ||
| Comments: | The H19-7 cell line was derived from hippocampi dissected from embryonic day 17 (E17) Holtzman rat embryos and immortalized by retroviral transduction of temperature sensitive tsA58 SV40 large T antigen. [49404 ] H19-7 cells grow at the permissive temperature (34C) in epidermal growth factor or serum. [48239 ] They differentiate to a neuronal phenotype at the nonpermissive temperature (39C) when induced by basic fibroblast growth factor (bFGF) in N2 medium (DMEM-high glucose medium with supplements). [48239 ] H19-7/IGF-IR cells were established by infecting H19-7 cells with a retroviral vector expressing the human type I insulin-like growth factor receptor (IGF-IR). The cells were selected in medium containing puromycin. [48239 ] H19-7/IGF-IR cells express the IGF-IR protein. IGF-IR is known to send two seemingly contradictory signals inducing either cell proliferation or cell differentiation, depending on cell type and/or conditions. At 39C, expression of the human IGF-IR in H19-7 cells induces an insulin-like growth factor (IGF) I dependent differentiation. The cells extend neurites and show increased expression of NF68. This cell line does not express detectable levels of the SV40 T antigen. This cell line can be used for analysis and comparison of mitogenesis (34C) and differentiation (39C). |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.2 mg/ml G418 and 0.001 mg/ml puromycin, 90%; fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 34.0°C Growth Conditions: Flasks used to propagate these cells must be coated with 0.015 mg/ml poly-l-lysine. Cover the surface of the vessel with poly-l-lysine solution for 5 minutes, remove and let dry. |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 48239: Morrione A, et al. Insulin-like growth factor I receptor signaling in differentiation of neuronal H19-7 cells. Cancer Res. 60: 2263-2272, 2000. PubMed: 10786694 49404: Eves EM, et al. Immortal rat hippocampal cell lines exhibit neuronal and glial lineages and neurotrophin gene expression. Proc. Natl. Acad. Sci. USA 89: 4373-4377, 1992. PubMed: 1316607 |
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文献和实验Examining Histone Acetlylation at Specific Genomic Regions
conceptuses to TSA leads to loss of H19 imprinting 3 . In X-chromosome inactivation, the inactive chromosome is associated with relatively underacetylated histones 4 . Changes in histone acetylation become apparent after the expression of Xist, the gene
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
pRB SBF (Swi4, Swi6) E2F-1, E2F-2 MBF (Mbp1, Swi6)
Mapping Networks of Protein‐Mediated Physical Interactions Between Chromatin Elements
Ling, J.Q., Li, T., Hu, J.F., Vu, T.H., Chen, H.L., Qiu, X.W., Cherry, A.M., and Hoffman, A.R. 2006. CTCF mediates interchromosomal colocalization between Igf2/H19 and Wsb1/Nf1. Science 312:269‐272.
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