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- 2026年06月27日
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- 器官来源：
脾
- 年限：
adult
- 生长状态：
悬浮生长
- 免疫类型：
IgG1 kappa
- 细胞形态：
淋巴样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- ATCC Number：
CRL-2567™
- 库存：
大量
| Designations: | H1.6 | ||
| Depositors: | J Dean | ||
| Isotype: | IgG1 kappa | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Organ: spleen Cell Type: hybridoma: lymphoblast B lymphocyte; somatic cell hybrid |
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| Cellular Products: | immunoglobulin; monoclonal antibody; against human ZP1 glycoprotein | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | ELISA detection zona pellucida glycoprotein 1 Western Blot zona pellucida glycoprotein 1 |
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| Age: | adult | ||
| Comments: | Animals were immunized with recombinant human zonae pellucidae 1 (ZP1). Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. An extracellular matrix that mediates critical steps in fertilization and early development surrounds all vertebrate eggs. In mice and humans, this matrix is known as the zona pellucida and comprises three glycoproteins: ZP1, ZP2 and ZP3. The H1.6 antibody specifically binds to human ZP1 glycoprotein. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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