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- 相关疾病：
腺癌
- ATCC Number：
CRL-1958™
- 年限：
31 years
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 器官来源：
宫颈
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
| Designations: | H1HeLa | ||
| Depositors: | RR Rueckert | ||
| Biosafety Level: | 2 [Cells contain human papilloma virus (HPV-18) ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: cervix Disease: adenocarcinoma |
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| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Reverse Transcript: | negative | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
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| Isoenzymes: | G6PD, A | ||
| Age: | 31 years | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| HeLa Markers: | Y | ||
| Comments: | The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2 ). This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses. The line is usually carried as monolayer cultures in Leibovitz's L-15 medium, but can be readily adapted to other media and to suspension culture (in media without Ca++). The H-HeLa line was cured of a mycoplasma contamination by W.M. Lee in Rueckert's laboratory and the cured line was given the designation H1-HeLa. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week Remove the medium and add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the culture to sit for 5 minutes at room temperature. Add fresh culture medium, aspirate and dispense into new flasks. |
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| References: | 21853: Lee WM Ph.D. thesis, Univ. Wisconsin, 1991 22695: Medappa KC, et al. On the structure of rhinovirus 1A. Virology 44: 259-270, 1971. PubMed: 4327717 |
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文献和实验HeLa is one of the oldest and most commonly used cell lines in biomedical research. Owing to the ease of which they can be effectively synchronized by various methods, HeLa cells have been used extensively for studies of the cell cycle
佚名 人工合成的H 1 受体阻断药多具有乙基胺的共同结构， Ar 1 和Ar 2 可为苯环或杂环，X可为氮、氧或碳，并与主核相联。乙基胺与组胺的侧链相似，对H 1 受体有较大亲和力，但无内在活性，故能竞争性阻断之。 【药理
Preparation of HeLa Nuclear Extracts
Promoter-specific in vitro transcription by RNA polymerase II is now achievable with cell extracts prepared from a variety of animal and plant sources (1 –3 ). For extracts prepared from mammalian cells, HeLa cells provide many advantages
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