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- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 细胞形态：
成纤维样
- ATCC Number：
CCL-93™
- 器官来源：
肺
- 物种来源：
仓鼠
- 是否是肿瘤细胞：
0
| Designations: | V79-4 | ||
| Depositors: | EH Chu | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Cricetulus griseus | ||
| Morphology: | fibroblast |
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| Source: | Organ: lung | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Cytogenetic Analysis: | modal number = 22; range = 20 to 23, Pseudodiploid. The rate of higher ploidies was 4%. Twelve to fourteen marker chromosomes were common to most cells. These include 1p-, 4q+, 4p+, t(6,?), 7q+ and seven to eight other small markers., Normal N2 and N3 were paired; N1, N5, N6 and N10 were single. Normal X and Y were absent, but a single Xq- was present in every cell. | ||
| Gender: | male | ||
| Comments: | This cell line was developed by Ford and Yerganian in 1958 from lung tissue of a young male Chinese hamster, and was originally designated Strain V. The line was renamed V79 by Elkind in 1958,and was used in studies of X-ray induced damage and repair processes. In 1966, E.H.Y. Chu obtained the line from W. Sinclair, and isolated the V79-4 clone. The cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours. A culture at passage 7 after cloning was submitted to the ATCC in July 1988. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:14 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 derivative:ATCC CRL-1657 |
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| References: | 21877: . Molecular cell genetics. New York: Wiley; 1985. 22642: Chu EH, et al. Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro. Genetics 62: 359-377, 1969. PubMed: 5392645 23252: Chu EH, Malling HV. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc. Natl. Acad. Sci. USA 61: 1306-1312, 1968. PubMed: 5249812 26264: . . Nature 184: 1293-1295, 1959. 26265: Ford DK, Yerganian G. Observations on the chromosomes of Chinese hamster cells in tissue culture. J. Natl. Cancer Inst. 21: 393-425, 1958. PubMed: 13576097 26275: . Molecular cell genetics. New York: Wiley; 1985. 32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 |
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文献和实验V79 Cytotoxicity Test for Membrane Damage
The cytotoxic effect of test chemicals in V79 cell culture can be determined by assessing damage to the plasma membrane as determined by a nucleic acid leakage assay.
【交流】体外哺乳类细胞（V79HGPRT）基因突变试验（GB）
中华人民共和国国家标准 GB 15193．12一91 体外哺乳类细胞（V79/HGPRT）基因突变试验 V79/HGPRT gene mutation test ------------------------------------------------------------------------- 1 主体内容与适用范围 1.1 本标准规定了体外哺乳类细胞（V79/HGPRT）基因突变试验的基本技术要求。 1.2 本标准适用于需要进行食品安全性评价的用作或用于
体外哺乳类细胞（V79/HGPRT）基因突变试验－ 中华仓鼠肺细胞染色体突变实验
药物毒理体外经典实验。国家都有标准了就可以想象重要性了。自己做了一个PDF文件，方便大家收藏。 中华人民共和国国家标准 GB 15193．12一91 体外哺乳类细胞（V79/HGPRT）基因突变试验 V79/HGPRT gene mutation test ------------------------------------------------------------------------- 1 主体内容与适用范围 1.1 本标准规定了体外
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