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- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- ATCC Number：
CRL-2467™
- 年限：
10 days
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
单核细胞/巨噬细胞
- 器官来源：
大脑
- 库存：
大量
- 品系：
C3H/HeJ
- 细胞类型：
其他细胞类型
| Designations: | EOC 2 | ||
| Depositors: | WS Walker | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus deposited as mouse | ||
| Morphology: | macrophage |
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| Source: | Organ: brain Strain: C3H/HeJ Cell Type: microglia; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | colony stimulating factor 1 (CSF-1R, CD115) | ||
| Antigen Expression: | CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD86 (B7.2) +; CD45 +, Ly-6C +, F4/80 +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR + [39974 ] | ||
| Age: | 10 days | ||
| Gender: | female | ||
| Comments: | This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. [39974 ] Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. The cell line is dependent on colony stimulating factor 1 (CSF-1). The cells exhibit phagocytic activity. Unlike EOC 13.31 (ATCC CRL-2468 ) and EOC 20 (ATCC CRL-2469 ), these cells do not constitutively express high levels of major histocompatibility complex (MHC) class II antigens and expression is not upregulated by recombinant murine interferon-gamma. The cells may be used to characterize the role of brain macrophages. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4) |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20% Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 39968: Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530 39974: Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814 39975: Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775 |
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文献和实验Isolation and Culture of Ovarian Cancer Cells and Cell Lines
carcinomas (EOC). EOC is often large and advanced at the time of presentation, so that cells are readily obtainable from surgical specimens or effusions. While the primary tumor may be chemosensitive, they often develop resistance and may do so rapidly
Establishment of Primary Cultures from Ovarian Tumor Tissue and Ascites Fluid
We have refined the technique for isolating and propagating cultures of primary epithelial ovarian cancer (EOC) cells derived from solid tumors and ascites. Both protocols involve a simple yet rapid method for the growth and propagation
Proteomic Profiling of Ovarian Cancer Models Using TMT-LC-MS/MS
Herein, we have utilized two cellular models of epithelial ovarian cancer (EOC), where transfer of normal chromosome 18 material into the EOC cell lines TOV-112D and TOV-21G induced in vitro and in vivo suppression of tumorigenic phenotype
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