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- 细胞形态：
单核细胞/巨噬细胞
- 品系：
C3H/HeJ
- 器官来源：
大脑
- 库存：
大量
- ATCC Number：
CRL-2469™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 细胞类型：
其他细胞类型
- 运输方式：
冻存运输
- 年限：
10 days juvenile
| Designations: | EOC 20 | ||
| Depositors: | WS Walker | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus deposited as mouse | ||
| Morphology: | macrophage |
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| Source: | Organ: brain Strain: C3H/HeJ Cell Type: microglia; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | colony stimulating factor 1 (CSF-1R, CD115) | ||
| Antigen Expression: | CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD45 +, Ly-6C +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR +, F4/80 +/-, CD86 (B7.2) - [39974 ] | ||
| Age: | 10 days juvenile | ||
| Gender: | female | ||
| Comments: | This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse [PubMed: 8550814]. Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. The cell line is dependent on growth factor colony stimulating factor 1 (CSF-1). Conditioned medium is made from LADMAC cells (ATCC CRL-2420?) as a source of CSF-1.The cells exhibit phagocytic activity. These cells constitutively expressed high levels of major histocompatibility complex (MHC) class II antigens and expression was upregulated by recombinant murine interferon-gamma. The cells may be used to characterize the role of brain macrophages.C3H/HeJ strain is defective in TLR4 (toll-like receptor 4) | ||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20% Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: Every 2 to 3 days Remove 75% of the media. Scrape off the attached cells and transfer into new flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 purified RNA:ATCC CRL-2469R |
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| References: | 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247 39968: Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530 39974: Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814 39975: Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775 |
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