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- 详细信息
- 文献和实验
- 技术资料
- 运输方式:
冻存运输
- 免疫类型:
IgG1
- 细胞形态:
淋巴样
- ATCC Number:
CRL-1644™
- 生长状态:
悬浮生长
- 库存:
大量
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
| Designations: | SJK-287-38 | ||
| Depositors: | D Korn | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human DNA polymerase alpha | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | H-2db | ||
| Comments: | Mice were immunized with fraction VIII human DNA polymerase alpha. Spleen cells were fused with NS-1 myeloma cells. The antibody neutralizes polymerase alpha activity, but does not react with DNA polymerases beta and gamma. Tested and found negative for ectromelia virus (mousepox). Formerly ATCC TIB-179. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml. |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 959: Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672 | ||
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文献和实验的维生素 D 状态是否与新冠检测阳性相关,并控制了人口统计学和共病指标。 其中,124 名参与者(25%)显示维生素 D 含量不足,287 名参与者(59%)维生素 D 含量充足。统计结果发现缺乏维生素 D 的人感染新冠的风险,比维生素 D 充足的人高出近两倍。 图 1:来源 JAMA Network Open 补充维生素 D,可降低新冠感染风险、改善新冠感染者的病情 2020 年 11 月,发表在 Postgrad Med J 杂志上的一项研究 Short term, high
~ 100 32 32.00 63 20 31.74 37 12 32.43 60~ 75 36 48.00 46 25 54.35 29 11 37.93 合计 1776 635 35.75 864 287 33.21 912 348 38.16 表2 黑龙江垦区东西部两地区自然人群Hp感染情况比较 年龄(岁) 东部地区 西部地区 总数 阳性数 阴性率(%) 总数 阳性数 阴性率(%) 0~ 313 69 22.0 49 17 34.7 10~ 168 64 38.1 117
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