- 询价
- 2026年06月19日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 器官来源：
结肠
- 库存：
大量
- 细胞形态：
上皮样
- 相关疾病：
肿瘤
- ATCC Number：
CRL-2577™
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
| Designations: | RKO | ||
| Depositors: | MC Hollander, AJ Fornace | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
||
| Source: | Organ: colon Disease: carcinoma |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | urokinase receptor (u-PAR) [51462 ] | ||
| Tumorigenic: | Yes | ||
| Oncogene: | p53 + (wild type) | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 8, 10 D13S317: 8, 11 D16S539: 12, 13 D5S818: 11, 13 D7S820: 8, 10 THO1: 6, 10 TPOX:11 vWA: 15, 16, 17 |
||
| Comments: | RKO is a poorly differentiated colon carcinoma cell line developed by Michael Brattain. RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor (h-TRbeta1). The level of p53 protein is higher in RKO (ATCC CRL-2577 ) cells than in RKO-E6 (ATCC CRL-2578 ) cells. [53331 ] The RKO cell line is the parental cell line (isogenic) of RKO-E6 (ATCC CRL-2578 ) and RKO-A545-1 (ATCC CRL-2579 ). It can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
||
| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 derivative:ATCC CRL-2578 derivative:ATCC CRL-2579 |
||
| References: | 51462: Boyd D, et al. Determination of the levels of urokinase and its receptor in human colon carcinoma cell lines. Cancer Res. 48: 3112-3116, 1988. PubMed: 2835152 51463: Smith ML, et al. Involvement of the p53 tumor suppressor in repair of u.v.-type DNA damage. Oncogene 10: 1053-1059, 1995. PubMed: 7700629 53329: Brattain MG, et al. Heterogeneity of human colon carcinoma. Cancer Metastasis Rev. 3: 177-191, 1984. PubMed: 6437669 53331: Bhat MK, et al. Tumor suppressor p53 is a negative regulator in thyroid hormone receptor signaling pathways. J. Biol. Chem. 272: 28989-28993, 1997. PubMed: 9360971 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验correct reconstruction of A53T and A30P.（8）结果:DNA测序结果证明构建载体插入正确α-突触核蛋白突变体A53T与A30P基因。（9）METHODS: RKO cells were treated with selective DNA methyltransferase (DNMTs) inhibitor, 5-Aza-2′-deoxycytidine (5-Aza-CdR), for 72 h. Methylation-specific PCR (MSP), T
) C2BBe1 [clone of Caco-2 (ATCC HTB-37)] CRL-2158 Homo sapiens (human) LS1034 CRL-2329 Homo sapiens (human) HCC1500 CRL-2343 Homo sapiens (human) HCC2218 CRL-2363 Homo sapiens (human) HCC2218 BL CRL-2577 Homo sapiens (human) RKO
肝癌细胞 400元 RKO TCHu116 人 结肠腺癌细胞 1000元 RKO-AS45-1 TCHu117 人 结肠癌转基因细胞 1000元 RKO-E6 TCHu118 人 结肠癌转基因细胞 1000元 RM-1 TCM14 小鼠 前列腺癌细胞 400元 S-180 TCM15 小鼠 腹水瘤细胞 400元 SAC-ⅡB2 TCM16 小鼠 腹水瘤细胞 400元 SAC-ⅡC3 TCM17 小鼠 腹水瘤细胞 400元
技术资料暂无技术资料 索取技术资料
