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- 详细信息
- 技术资料
- 器官来源：
肾脏
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
上皮细胞
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2947™
- 年限：
6 weeks
- 品系：
Balb/cCr
| Designations: | Renca | ||
| Depositors: | R.Wiltrout | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | epithelial-like |
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| Source: | Organ: kidney Disease: renal adenocarcinoma Cell Type: epithelial Strain: Balb/cCr |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1969 | ||
| Tumorigenic: | YES | ||
| Age: | 6 weeks | ||
| Gender: | male | ||
| Comments: | The Renca cell line was derived from a tumor that arose spontaneously as a renal cortical adenocarcinoma in Balb/cCr mice. The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver. [PubMed: 4703766, 4057425]The cells do not express transforming growth factor-beta type II receptor (TbetaR-II). [PubMed: 10414746] | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Preservation: | Freeze medium: RPMI-1640 Medium, 77.5%; FBS, 15% FBS; DMSO, 7.5% Storage temperature liquid nitrogen vapor phase |
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| Doubling Time: | approximately 24 hours | ||
| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001 Recommended serum: ATCC 30-30-2020 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Phosphate-buffered saline: ATCC 30-2200 Cell culture tested DMSO: ATCC 4-X L-Glutamine solution, 200mM: ATCC 30-2214 Erythrosin B vital stain solution: ATCC 30-2404 |
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| References: | 16172681: Murphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766 16172682: Salup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425 16172683: Engel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746 |
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Renca
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