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- 2025年10月12日
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- 细胞形态：
成纤维样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 相关疾病：
肿瘤
- 器官来源：
其他
- ATCC Number：
CRL-1734™
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
| Designations: | SCA-9 clone 15 | ||
| Depositors: | T Barka | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: submandibular salivary gland Disease: carcinoma |
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| Cellular Products: | epidermal growth factor (EGF); nerve growth factor (NGF); renin; peptide hydrolase | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Gender: | male | ||
| Comments: | This is a soft agar clone of the SCA 124-9 cell line. The parental line was derived from an undifferentiated carcinoma of the submandibular gland induced by intraglandular injection of 7,12-dimethylbenz[a]anthracene. The line produces low levels of several factors (e.g., EGF, NGF). Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 1595: Barka T, et al. Epidermal growth factor, renin, and peptidase in cultured tumor cells of submandibular gland origin. Lab. Invest. 42: 656-662, 1980. PubMed: 6993784 | ||
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