- 询价
- 2025年12月08日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 细胞形态：
淋巴样
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 年限：
36 years
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1964™
- 生长状态：
悬浮生长
- 器官来源：
外周血
| Designations: | Clone 15 HL-60 | ||
| Depositors: | S Fischkoff | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
||
| Source: | Organ: peripheral blood Disease: acute promyelocytic leukemia |
||
| Cellular Products: | eosinophil granule proteins: eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1984 | ||
| Receptors: | complement, expressed Fc, expressed interleukin 5 (IL-5), expressed |
||
| Reverse Transcript: | negative | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 13,14 D13S317: 8,11 D16S539: 11 D5S818: 12 D7S820: 11,12 THO1: 7,8 TPOX: 8,11 vWA: 16 |
||
| Age: | 36 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240 ) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months. This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with butyric acid. Initially, 80% of the treated cells will differentiate as eosinophils. However, with continued passage the line will gradually exhibit a modest degree of reversion such that a higher percentage of the cells will exhibit neutrophilic differentiation. The cells express IL-5 receptors and eosinophil granule proteins, and exhibit chemotaxis to eosinophil specific eosinophilotactins. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
||
| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of medium. Start new cultures at 2 X 10(5) viable cells/ml and subculture at 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
||
| References: | 22787: Fischkoff SA. Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions. Leuk. Res. 12: 679-696, 1988. PubMed: 3184985 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Single Clone Excision Protocol
. 4.In a 15 ml conical tube,place 200 ml XL1-Blue MRF' cells (at A600 of 1.0; if absorbance differs,adjust amount proportionally) 250 ml of phage stock (from step 1 above.Make sure that you don't transfer chloroform from the bottom of the tube). 1 ml
Clone Genes From a Phage Library
much more complicated than it actually is. First you must have the SolR cells grown up and prepared for phage plating, and get out some Exassist phage as well from the freezer. • In a 15 ml falcon tube, combine 200 microliters XL blue cells and 200
Clone information and RNAi Feeding Protocol
AHRINGER LAB Clone information and RNAi Feeding Protocol Vector and inserts: Genomic fragments obtained by PCR were cloned into the Timmons and Fire feeding vector (L4440
技术资料暂无技术资料 索取技术资料
