- 询价
- 2026年03月01日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 免疫类型：
IgM
- 细胞形态：
淋巴样
- ATCC Number：
HB-166™
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
| Designations: | 2.28 M1 [A2,28M1] | ||
| Depositors: | F Grumet | ||
| Isotype: | IgM | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4). Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for a subpopulation of HLA A2 positive cells and HLA A28 positive cells at an appropriate titer. The antibody recognizes an epitope at the amino terminal end of the alpha 2 domain of the HLA-A2 molecule. [39936 ] Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666 | ||
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文献和实验： 取16.28硅胶H, 1.8 g MN30OUV 254纤维素，0.5外挟甲基纤维素钠(离心去除悬浮物)90ml，于匀浆器中匀浆5分钟，速度以浆液不溅出为度，抽气，均匀涂布在6块20× l 0 cm或4块20×1 5cm的平整清洁的玻板上。 (2)薄层分离：称取25X103nM (1nM二10-9 M)A ,G ,C ,T ,U ,盛于不同容器中，再分别称取同样量的上述各碱基混合于一个容器，各加Im l水，1滴12N NaOH 液助溶，后加1滴浓HCl，用0.1 N HCI稀释到5m1
最好为新鲜的，或者在－70℃条件下保存半年以下；尽量避免材料的冻融 。 3、确认RNA的质量 检测RNA溶液的吸光度：OD260/OD280=1.8－2.0 RNA的电泳图谱：28S和18S条带明亮、清晰、指条带边缘清晰，并且28S的亮度在18S条带的两倍以上。 二、反转录： 单管一步法: 反转录和PCR反应在一个管子中完成 ，得到的全部cDNA产物都一起经PCR扩增 ，灵敏度更高，但是容易相互干扰 。 两步法: 反转录和P CR分开做，PCR取反转录反应
)、刻度试管5.0m1(×18)、 吸管0.10mL(×l)、0.50mL(×4)、l.0mL(×2)、 5.0m1(×l)、电子天平、722 型(或7220 型)分光光度计、容量瓶10mL(×l)。 原料 卵清蛋白或其他蛋白质样品 试剂 1. 2％卵清蛋白液：称取0.5g 卵清蛋白，溶于蒸馏水①(为了减少蛋白质变性，溶解时只能用玻棒轻缓搅动。)离心去除不溶物，取上清液加水稀释至25mL 。 2. 尿素(A.R.)：粉末状。 3. 半恍氨
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