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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 相关疾病：
其他疾病
- ATCC Number：
TIB-192™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 品系：
SL
| Designations: | M1 | ||
| Depositors: | P Ralph | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | |
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| Source: | Disease: myeloid leukemia Strain: SL Cell Type: myeloblast; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Established in vitro from spontaneous myeloid leukemia of SL strain mice. Can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone, LPS and WEHI-3 conditioned medium. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
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| References: | 1220: Ralph P, et al. Expression and induction in vitro of macrophage differentiation antigens on murine cell lines. J. Immunol. 130: 108-114, 1983. PubMed: 6847876 22710: Ichikawa Y. Differentiation of a cell line of myeloid leukemia. J. Cell. Physiol. 74: 223-234, 1969. PubMed: 4187945 33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 |
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文献和实验33及CD13阳性，CD11b、CD15阴性。CD33阳性者CR率高，CD13阳性、CD33阴性者CR率低。 5.染色体和分子生物学检验：核型异常、Ph染色体t（9；22）形成BCR-ABL融合基因，约见于3%的AML，大多为M1型。
Studying M1 and M2 States in Adult Microglia
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Liquid Chromatographic Method for Aflatoxin M1 in Milk
Aflatoxin M1 is the 4-hydroxy derivative of aflatoxin B1 (see Fig. 1 ). Aflatoxin M1 appears in milk and milk products as the direct result of the intake of aflatoxin B1 -contaminated feed by dairy cows
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